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Groups of sea lamprey (Petromyzon marinus) eggs fertilized by males previously immersed in an aqueous solution of p,p-bis (1-aziridinyl)-N-methylphosphinothioic amide (bisazir) at concentrations of 10-100 mg/L produced fewer normal, live prolarvae after 15-17 d of incubation than did groups of eggs fertilized by normal males. Mortality of embryos or prolarvae was nearly 100% in groups of eggs fertilized by males that had been immersed in a 50 mg/L solution of bisazir for 4 h or in a 100 mg/L solution for 2 h. The immersion technique appears to be an efficient method of sterilizing large numbers of male sea lampreys for use in a proposed sterile-male-release program.
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Sterilization of sea lampreys (Petromyzon marinus) by immersion in an aqueous solution of bisazir
Canadian Journal of Fisheries and Aquatic Sciences