A revised annotated checklist for the chironomid midges (Diptera: Chironomidae) of the southeastern United States is presented that includes the states of Alabama, Florida, Georgia, North Carolina, South Carolina, and Tennessee. Much of the information concerns occurrence and habitat preference records based upon the authors' data, as well as published and unpublished data. Some information is also presented that includes aspects of biology, habitat preference, bibliographic sources, and nomenclatorial changes. Based upon the present work, the chironomid fauna of the southeastern states is comprised of 189 genera (172 described, 17 informally or unofficially described) and 754 species (505 described, 17 informally or unofficially described, 33 that are assumed for generic or subgeneric presence only, 197 estimated species, and 2 species groups). Several new species synonyms and generic placements are recognized. Thirty-eight genera known from the Nearctic region remain unknown from the southeastern states. Diversity of species was greatest in the subfamily Chironominae, considering named as well as unnamed and estimated species. There were no significant changes in overall regional distribution patterns of subfamilies or habitat preferences form that which has been previously reported. The greatest totals for regional records, habitat types, and state occurrences were the Coastal Plain (378), streams (271), and North Carolina (373), respectively.