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Optimization of a liquid chromatographic method for determination of malachite green and its metabolites in fish tissues

Journal of AOAC International

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Abstract

A liquid chromatographic (LC) method was adapted and optimized for the determination of malachite green and its metabolites in fish plasma and muscle, Residues in plasma were extracted with acetonitrile, the extract was evaporated to dryness, and residues were resolubilized for LC analysis, Residues in muscle were extracted with an acetonitrile-acetate buffer mixture, reextracted with acetonitrile, and partitioned into methylene chloride with final cleanup on alumina and propylsulfonic acid solid-phase extraction columns, Residue levels were determined by using an LC cyano column with a PbO2 postcolumn and visible detection (618 nm). Overall mean recoveries of parent malachite green (MG-C) and its major metabolite, leucomalachite green (MG-L), from plasma were 93 and 87%, respectively, at fortification levels ranging from 25 to 250 ppb, Overall mean recoveries of MG-C and MG-L from muscle were 85 and 95%, respectively, at fortification levels ranging from 5 to 100 ppb, Relative standard deviations (RSDs) of recoveries at all fortification levels ranged from 3.9 to 7.0% for plasma and from 2.1 to 5.2% for muscle, The method was applied to incurred residues in tissues sampled from catfish after waterborne exposure to [C-14]MG-C. Mean recoveries of total radioactive residues in plasma and muscle throughout the extraction and cleanup process were 88 and 87%, respectively, and corresponding RSDs for MG-C and MG-L were in the same range as those for fortified tissues, MG-L, was confirmed as the major metabolite of MG-C in catfish.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Optimization of a liquid chromatographic method for determination of malachite green and its metabolites in fish tissues
Series title:
Journal of AOAC International
Volume
78
Issue:
6
Year Published:
1995
Language:
English
Publisher:
US FDA Gulf Coast Seafood Lab
Publisher location:
Dauphin Island, AL
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
pp. 1388-1394
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of AOAC International
First page:
1388
Last page:
1394
Number of Pages:
7