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Liquid chromatographic determination of oxytetracycline in edible fish fillets from six species of fish

Journal of AOAC International

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Abstract

The approved use of oxytetracycline (OTC) in U.S. Aquaculture is limited to specific diseases in salmonids and channel catfish. OTC may also be effective in controlling diseases in other fish species important to public aquaculture, but before approved use of OTC can be augmented, an analytical method for determining OTC in fillet tissue from multiple species of fish will be required to support residue depletion studies. The objective of this study was to develop and validate a liquid chromatographic (LC) method that is accurate, precise, and sensitive for OTC in edible fillets from multiple species of fish. Homogenized fillet tissues from walleye, Atlantic salmon, striped bass, white sturgeon, rainbow trout, and channel catfish were fortified with OTC at nominal concentrations of 10, 20, 100, 1000, and 5000 ng/g. In tissues fortified with OTC at 100, 1000, and 5000 ng/g, mean recoveries ranged from 83 to 90%, and relative standard deviations (RSDs) ranged from 0.9 to 5.8%. In all other tissues, mean recoveries ranged from 59 to 98%, and RSDs ranged from 3.3 to 20%. Method quantitation limits ranged from 6 to 22 ng/g for the 6 species. The LC parameters produced easily integratable OTC peaks without coelution of endogenous compounds. The method is accurate, precise, and sensitive for OTC in fillet tissue from 6 species of fish from 5 phylogenetically diverse groups.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Liquid chromatographic determination of oxytetracycline in edible fish fillets from six species of fish
Series title:
Journal of AOAC International
Volume
81
Issue:
4
Year Published:
1998
Language:
English
Publisher:
U.S. Geological Survey
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
pp. 702-708
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of AOAC International
First page:
702
Last page:
708
Number of Pages:
7