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Reversed-phase thin-layer chromatography of homologs of Antimycin-A and related derivatives

Journal of Chromatography

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Abstract

Using a reversed-phase high-performance liquid chromatographic (HPLC) technique, a mixture of antimycins A was separated into eight hitherto unreported subcomponents, Ala, Alb, A2a, A2b, A3a, A3b, A4a, and A4b. Although a base-line resolution of the known four major antimycins Al, A2, A3, and A4 was readily achieved with mobile phases containing acetate buffers, the separation of the new antibiotic subcomponents was highly sensitive to variation in mobile phase conditions. The type and composition of organic modifiers, the nature of buffer salts, and the concentration of added electrolytes had profound effects on capacity factors, separation factors, and peak resolution values. Of the numerous chromatographic systems examined, a mobile phase consisting of methanol-water (70:30) and 0.005 M tetrabutylammonium phosphate at pH 3.0 yielded the most satisfactory results for the separation of the subcomponents. Reversed-phase gradient HPLC separation of the dansylated or methylated antibiotic compounds produced superior chromatographic characteristics and the presence of added electrolytes was not a critical factor for achieving separation. Differences in the chromatographic outcome between homologous and structural isomers were interpretated based on a differential solvophobic interaction rationale. Preparative reversed-phase HPLC under optimal conditions enabled isolation of pure samples of the methylated antimycin subcomponents for use in structural studies.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Reversed-phase thin-layer chromatography of homologs of Antimycin-A and related derivatives
Series title:
Journal of Chromatography
Volume
464
Issue:
2
Year Published:
1989
Language:
English
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
453-458
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Chromatography
First page:
453
Last page:
458