| Abstract: | Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteins were measured in pipping black-crowned night heron (Nycticorax nycticorax) embryos collected from a reference site (next to the Chincoteague National Wildlife Refuge, VA) and three polluted sites (Cat Island, Green Bay, Lake Michigan, WI; Bair Island, San Francisco Bay, CA; West Marin Island, San Francisco Bay, CA). In a laboratory study, artificially incubated night heron embryos from the reference site were treated with 3-methylcholanthrene (200 mu g administered into the air cell 2 d before pipping) or phenobarbital (2 mg daily for 2 d before pipping). Compared to controls (untreated + vehicle-treated embryos), 3-methylcholanthrene induced a greater than fivefold increase in activities of several monooxygenases (arylhydrocarbon hydroxylase, AHH; benzyloxyresorufin-O-dealkylase, BROD; ethoxyresorufin-O-dealkylase, EROD; pentoxyresorufin-O- dealkylase, PROD) and a greater than 100-fold increase in the concentration of immunodetected cytochrome P450 1A (CYP1A). Phenobarbital treatment resulted in only a slight increase in BROD activity but induced proteins recognized by antibodies to cytochrome P450 2B (CYP2B) by 2,000-fold. In a field study, activities of AHH, BROD, EROD, and ethoxycoumarin-O-dealkylase (ECOD) were up to 85-fold higher in pipping black- crowned night herons collected from Cat Island compared to other sites. Hepatic CYP1A and CYP2B cross- reactive proteins were detected in significantly more individuals from Cat Island than from the reference site. Greatest burdens of total PCBs and p,p‘-DDE were detected in embryos from Cat Island. Cytochrome P450- associated monooxygenase activities and cytochrome P450 proteins (AHH, BROD, EROD, ECOD, CYP1A, CYP2B) were significantly associated with total PCB burdens (r = 0.50-0.72). These data indicate that cytochrome P450 may be a useful biomarker of exposure to some PCB mixtures in black-crowned night heron embryos. |
| Genre: | Article |
| ProdID: | 1003191 |
| Citation Author: | Rattner, B. A.; Melancon, M. J.; Custer, T. W.; Hothem, R. L.; King, K. A.; LeCaptain, L. J.; Spann, J. W.; Woodin, B. R.; Stegeman, J. J. |
| Citation Contributing Office: | Upper Midwest Environmental Sciences Center |
| Citation Datum: | |
| Citation Day: | |
| Citation Edition: | |
| Citation Editor: | |
| Citation End Page: | 1732 |
| Citation Issue: | 9 |
| Citation Keywords: | |
| Citation Language: | English |
| Citation Larger Work Title: | Environmental Toxicology and Chemistry |
| Citation LatN: | |
| Citation LatS: | |
| Citation LonE: | |
| Citation LonW: | |
| Citation Month: | |
| Citation No Pagination: | |
| Citation Number Of Pages: | 14 |
| Citation Online Only Flag: | |
| Citation Phsyical Description: | pp. 1719-1732 |
| Citation Projection: | |
| Citation Public Comments: | |
| Citation Publisher: | U.S. Fish and Wildlife Service |
| Citation Series: | |
| Citation Series Code: | |
| Citation Series Number: | |
| Citation Search Results Text: | Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450; 1993; Article; Journal; Environmental Toxicology and Chemistry; Rattner, B. A.; Melancon, M. J.; Custer, T. W.; Hothem, R. L.; King, K. A.; LeCaptain, L. J.; Spann, J. W.; Woodin, B. R.; Stegeman, J. J. |
| Citation Start Page: | 1719 |
| Citation Volume: | 12 |
| Citation Year: | 1993 |
| Type: | citation/reference |
| Text: | Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450; 1993; Article; Journal; Environmental Toxicology and Chemistry; Rattner, B. A.; Melancon, M. J.; Custer, T. W.; Hothem, R. L.; King, K. A.; LeCaptain, L. J.; Spann, J. W.; Woodin, B. R.; Stegeman, J. J. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Fri, 1 Jan 1993 00:00 -0600 |
| Publisher: | U.S. Fish and Wildlife Service |
