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Screening fungicides for use in fish culture: Evaluation of the agar plug transfer, cellophane transfer, and agar dilution methods

Progressive Fish-Culturist

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Abstract

The reliability, reproducibility, and usefulness of three screening methods -- the cellophane transfer, the agar plug transfer, and the agar dilution -- to screen aquatic fungicides were evaluated. Achlya flagellata and Saprolegnia hypogyna were exposed to 1, 10, and 100 mg/L of malachite green to test each method. The cellophane transfer and agar plug transfer techniques had similar reliability and reproducibility in rating fungicidal activity, and were both superior to the agar dilution technique. The agar plug transfer and agar dilution techniques adequately projected in vivo activity of malachite green, but the cellophane transfer technique overestimated its activity. Overall, the agar plug transfer technique most accurately rated the activity of malachite green and was the easiest test to perform. It therefore appears to be the method of choice for testing aquatic fungicides.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Screening fungicides for use in fish culture: Evaluation of the agar plug transfer, cellophane transfer, and agar dilution methods
Series title:
Progressive Fish-Culturist
Volume
45
Issue:
1
Year Published:
1983
Language:
English
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
pp. 24-27
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Progressive Fish-Culturist
First page:
24
Last page:
27
Number of Pages:
4