| Abstract: | A high-performance liquid chromatography (HPLC) procedure that is rapid, specific, and sensitive (limit of detection <0.005 mg/liter) was developed for monitoring application and degradation rates of rotenone. For analysis, a water sample is buffered to pH 5 and injected through a Sep Pak(R) C18 disposable cartridge. The cartridge adsorbs and retains the rotenone which then can be eluted quantitatively from the cartridge with a small volume of methanol. This step effectively concentrates the sample and provides sample cleanup. The methanol extract is analyzed directly by HPLC on an MCH 10 reverse-phase column; methanol: water (75:25, volume : volume) is the mobile phase and flow rate is 1.5 ml/minute. The rotenone is detected by ultraviolet spectrophotometry at a wavelength of 295 nm. |
| Genre: | Article |
| ProdID: | 1003319 |
| Citation Author: | Dawson, V. K.; Harman, P. D.; Schultz, D. P.; Allen, J. L. |
| Citation Contributing Office: | Upper Midwest Environmental Sciences Center |
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| Citation End Page: | 727 |
| Citation Issue: | 5 |
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| Citation Language: | English |
| Citation Larger Work Title: | Transactions of the American Fisheries Society |
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| Citation Number Of Pages: | 3 |
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| Citation Phsyical Description: | pp. 725-727 |
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| Citation Search Results Text: | Rapid method for measuring rotenone in water at piscicidal concentrations; 1983; Article; Journal; Transactions of the American Fisheries Society; Dawson, V. K.; Harman, P. D.; Schultz, D. P.; Allen, J. L. |
| Citation Start Page: | 725 |
| Citation Volume: | 112 |
| Citation Year: | 1983 |
| Type: | citation/reference |
| Text: | Rapid method for measuring rotenone in water at piscicidal concentrations; 1983; Article; Journal; Transactions of the American Fisheries Society; Dawson, V. K.; Harman, P. D.; Schultz, D. P.; Allen, J. L. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Sat, 1 Jan 1983 00:00 -0600 |
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