thumbnail

Rapid method for measuring rotenone in water at piscicidal concentrations

Transactions of the American Fisheries Society

By:
, , ,

Links

  • The Publications Warehouse does not have links to digital versions of this publication at this time

Abstract

A high-performance liquid chromatography (HPLC) procedure that is rapid, specific, and sensitive (limit of detection <0.005 mg/liter) was developed for monitoring application and degradation rates of rotenone. For analysis, a water sample is buffered to pH 5 and injected through a Sep Pak(R) C18 disposable cartridge. The cartridge adsorbs and retains the rotenone which then can be eluted quantitatively from the cartridge with a small volume of methanol. This step effectively concentrates the sample and provides sample cleanup. The methanol extract is analyzed directly by HPLC on an MCH 10 reverse-phase column; methanol: water (75:25, volume : volume) is the mobile phase and flow rate is 1.5 ml/minute. The rotenone is detected by ultraviolet spectrophotometry at a wavelength of 295 nm.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Rapid method for measuring rotenone in water at piscicidal concentrations
Series title:
Transactions of the American Fisheries Society
Volume
112
Issue:
5
Year Published:
1983
Language:
English
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
pp. 725-727
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
725
Last page:
727
Number of Pages:
3