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Induction of sporulation and the influence of time, temperature, and inoculum size on growth in two species of aquatic fungi (Saprolegniales)

Journal of the Elisha Mitchell Scientific Society

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Abstract

Because the infective stage of most mycoses of aquatic organisms is the zoospore, we attempted to establish optimum conditions under which zoospores could be produced for use in antifungal testing. Optimum sporulation time, incubation time, inoculum size, and growth temperature were determined for each oftwo saprolegniaceous fungi, Achlya flagellata Coker and Saprolegnia hypogyna (Pringsheim) de Bary. Both species produced the largest number of zoospores after 18 hours (51.7 spores/ml for A. jlagellata and 848.0 spores/ml for S. hypogyna), and yielded maximum growth after 48 hours at 22 'C. The recommended test inoculum size for S. hypogyna (5,600 spores/ml was nearly three times that for A. flagellata (2,000 spores/ml),

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Induction of sporulation and the influence of time, temperature, and inoculum size on growth in two species of aquatic fungi (Saprolegniales)
Series title:
Journal of the Elisha Mitchell Scientific Society
Volume
106
Issue:
3
Year Published:
1990
Language:
English
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
pp. 57-63
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of the Elisha Mitchell Scientific Society
First page:
57
Last page:
63
Number of Pages:
7