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Avian leucocyte counting using the hemocytometer

Journal of Zoo and Wildlife Medicine

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Abstract

Automated methods for counting leucocytes in avian blood are not available because of the presence of nucleated erythrocytes and thrombocytes. Therefore, total white blood cell counts are performed by hand using a hemocytometer. The Natt and Herrick and the Unopette methods are the most common stain and diluent preparations for this procedure. Replicate hemocytometer counts using these two methods were performed on blood from four birds of different species. Cells present in each square of the hemocytometer were counted. Counting cells in the corner, side, or center hemocytometer squares produced statistically equivalent results; counting four squares per chamber provided a result similar to that obtained by counting nine squares; and the Unopette method was more precise for hemocytometer counting than was the Natt and Herrick method. The Unopette method is easier to learn and perform but is an indirect process, utilizing the differential count from a stained smear. The Natt and Herrick method is a direct total count, but cell identification is more difficult.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Avian leucocyte counting using the hemocytometer
Series title:
Journal of Zoo and Wildlife Medicine
Volume
25
Issue:
3
Year Published:
1994
Language:
English
Contributing office(s):
National Wildlife Health Center
Description:
p. 432-437
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Zoo and Wildlife Medicine
First page:
432
Last page:
437