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Quantitative analysis of herpes virus sequences from normal tissue and fibropapillomas of marine turtles with real-time PCR

Virology

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Abstract

Quantitative real-time PCR has been used to measure fibropapilloma-associated turtle herpesvirus (FPTHV) pol DNA loads in fibropapillomas, fibromas, and uninvolved tissues of green, loggerhead, and olive ridley turtles from Hawaii, Florida, Costa Rica, Australia, Mexico, and the West Indies. The viral DNA loads from tumors obtained from terminal animals were relatively homogenous (range 2a??20 copies/cell), whereas DNA copy numbers from biopsied tumors and skin of otherwise healthy turtles displayed a wide variation (range 0.001a??170 copies/cell) and may reflect the stage of tumor development. FPTHV DNA loads in tumors were 2.5a??4.5 logs higher than in uninvolved skin from the same animal regardless of geographic location, further implying a role for FPTHV in the etiology of fibropapillomatosis. Although FPTHV pol sequences amplified from tumors are highly related to each other, single signature amino acid substitutions distinguish the Australia/Hawaii, Mexico/Costa Rica, and Florida/Caribbean groups.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Quantitative analysis of herpes virus sequences from normal tissue and fibropapillomas of marine turtles with real-time PCR
Series title:
Virology
Volume
287
Issue:
1
Year Published:
2001
Language:
English
Contributing office(s):
National Wildlife Health Center
Description:
p. 105-111
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Virology
First page:
105
Last page:
111