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Determination of the median toxic dose of type C botulism in lactating dairy cows

Journal of Veterinary Diagnostic Investigation

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Abstract

Because of the difficulty in identifying botulinum toxin in cattle, it is hypothesized that cattle are sensitive to levels of toxin below the detection limits of current diagnostic techniques (the mouse protection bioassay and the immunostick enzyme-linked immunosorbent assay [ELISA] for type C botulinum toxin). Using an up-down method for toxicologic testing, the median toxic dose (MTD50) for cattle was determined. Four lactating Holstein cows were dosed at 0.125 or 0.25 ng/kg with Clostridium botulinum type C toxin and failed to develop clinical signs of botulism during the 7-day observation period. Three cows given 0.50 ng/kg of toxin developed clinical signs of botulism. From these results, the MTD50 was calculated at 0.388 ng/kg (3.88 mouse lethal doses/kg) using the trim-logit method. These results suggest that cattle are 12.88 times more sensitive to type C botulinum toxin than a mouse on a per kilogram weight basis. The mouse protection bioassay and the immunostick ELISA for type C botulinum toxin failed to identify the presence of the toxin in the serum, blood, and milk samples taken from all 7 animals.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Determination of the median toxic dose of type C botulism in lactating dairy cows
Series title:
Journal of Veterinary Diagnostic Investigation
Volume
15
Issue:
6
Year Published:
2003
Language:
English
Contributing office(s):
National Wildlife Health Center
Description:
p. 523-526
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Veterinary Diagnostic Investigation
First page:
523
Last page:
526
Number of Pages:
4