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Comparison of methods to detect Pasteurella multocida in carrier waterfowl

Journal of Wildlife Diseases

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Abstract

We conducted laboratory challenge trials using mallard ducks (Anas platyrhynchos) to compare methods for detecting carriers of Pasteurella multocida, the bacterium that causes avian cholera, in wild birds. Birds that survived the initial infection were euthanized at 2-4 wk intervals up to 14 wk post challenge. Isolates of P. multocida were obtained at necropsy from 23% of the birds that survived initial infection. We found that swab samples (oral, cloacal, nasal, eye, and leg joint) were most effective for detecting carrier birds up to 14 wk post infection. No detectable differences in isolation were observed for samples stored in either 10% dimethysulfoxide or brain heart infusion broth. The frequency of detecting carriers in our challenge trials appeared to be related to mortality rates observed during the trial, but was not related to a number of other factors including time after challenge, time delays in collecting tissues postmortem, and route of infection. In our trials, there was little association between antibody levels and carrier status. We concluded that swabs samples collected from recently dead birds, stored in liquid nitrogen, and processed using selective broth provide a feasible field method for detecting P. multocida carriers in wild waterfowl.

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Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Comparison of methods to detect Pasteurella multocida in carrier waterfowl
Series title:
Journal of Wildlife Diseases
Volume
39
Issue:
1
Year Published:
2003
Language:
English
Contributing office(s):
National Wildlife Health Center
Description:
p. 125-135
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Wildlife Diseases
First page:
125
Last page:
135
Country:
United States
State:
California, Nebraska
Online Only (Y/N):
N
Additional Online Files(Y/N):
N