| Abstract: | Fibropapillomatosis (FP) of marine turtles is a neoplastic disease of ecological concern. A fibropapilloma-associated turtle herpesvirus (FPTHV) is consistently present, usually at loads exceeding one virus copy per tumor cell. DNA from an array of parasites of green turtles (Chelonia mydas) was examined with quantitative PCR (qPCR) to determine whether any carried viral loads are sufficient to implicate them as vectors for FPTHV. Marine leeches (Ozobranchus spp.) were found to carry high viral DNA loads; some samples approached 10 million copies per leech. Isopycnic sucrose density gradient/qPCR analysis confirmed that some of these copies were associated with particles of the density of enveloped viruses. The data implicate the marine leech Ozobranchus as a mechanical vector for FPTHV. Quantitative RT-PCR analysis of FPTHV gene expression indicated that most of the FPTHV copies in a fibropapilloma have restricted DNA polymerase expression, suggestive of latent infection. |
| Genre: | Article |
| ProdID: | 1003732 |
| Citation Author: | Greenblatt, R. J.; Work, T. M.; Balazs, G.; Sutton, C. A.; Casey, R. N.; Casey, J. W. |
| Citation Contributing Office: | National Wildlife Health Center |
| Citation Datum: | |
| Citation Day: | |
| Citation Edition: | |
| Citation Editor: | |
| Citation End Page: | 110 |
| Citation Issue: | 1 |
| Citation Keywords: | |
| Citation Language: | English |
| Citation Larger Work Title: | Virology |
| Citation LatN: | |
| Citation LatS: | |
| Citation LonE: | |
| Citation LonW: | |
| Citation Month: | |
| Citation No Pagination: | |
| Citation Number Of Pages: | 10 |
| Citation Online Only Flag: | |
| Citation Phsyical Description: | p. 101-110 |
| Citation Projection: | |
| Citation Public Comments: | |
| Citation Publisher: | |
| Citation Series: | |
| Citation Series Code: | |
| Citation Series Number: | |
| Citation Search Results Text: | The Ozobranchus leech as a mechanical vector for the fibropapilloma-associated turtle herpesvirus found latently infecting skin tumors on Hawaiian green turtles (Chelonia mydas); 2004; Article; Journal; Virology; Greenblatt, R. J.; Work, T. M.; Balazs, G.; Sutton, C. A.; Casey, R. N.; Casey, J. W. |
| Citation Start Page: | 101 |
| Citation Volume: | 321 |
| Citation Year: | 2004 |
| Type: | citation/reference |
| Text: | The Ozobranchus leech as a mechanical vector for the fibropapilloma-associated turtle herpesvirus found latently infecting skin tumors on Hawaiian green turtles (Chelonia mydas); 2004; Article; Journal; Virology; Greenblatt, R. J.; Work, T. M.; Balazs, G.; Sutton, C. A.; Casey, R. N.; Casey, J. W. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (INDEX PAGE): | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WXR-4BV4VKV-2&_user=696292&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000038819&_version=1&_urlVersion=0&_userid=696292&md5=dd0697b5db2ab56f67f833bd8f11d1c8 |
| Date Other: | Thu, 1 Jan 2004 00:00 -0600 |
| Publisher: | |
