| Abstract: | A cryopreservation method and improved isolation techniques for detection of Pasteurella multocida from wetland samples were developed. Wetland water samples were collected in the field, diluted in dimethyl sulfoxide (DMSO, final concentration 10%), and frozen at -180 C in a liquid nitrogen vapor shipper. Frozen samples were transported to the laboratory where they were subsequently thawed and processed in Pasteurella multocida selective broth (PMSB) to isolate P. multocida. This method allowed for consistent isolation of 2 to 18 organisms/ml from water seeded with known concentrations of P. multocida. The method compared favorably with the standard mouse inoculation method and allowed for preservation of the samples until they could be processed in the laboratory. |
| Genre: | Article |
| ProdID: | 1003985 |
| Citation Author: | Moore, Melody K.; Shadduck, D. J.; Goldberg, D. R.; Samuel, M. D. |
| Citation Contributing Office: | National Wildlife Health Center |
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| Citation End Page: | 185 |
| Citation Issue: | 1 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of Wildlife Diseases |
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| Citation Number Of Pages: | 4 |
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| Citation Phsyical Description: | p. 182-185 |
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| Citation Search Results Text: | A cryopreservation method for Pasteurella multocida from wetland samples; 1998; Article; Journal; Journal of Wildlife Diseases; Moore, Melody K.; Shadduck, D. J.; Goldberg, D. R.; Samuel, M. D. |
| Citation Start Page: | 182 |
| Citation Volume: | 34 |
| Citation Year: | 1998 |
| Type: | citation/reference |
| Text: | A cryopreservation method for Pasteurella multocida from wetland samples; 1998; Article; Journal; Journal of Wildlife Diseases; Moore, Melody K.; Shadduck, D. J.; Goldberg, D. R.; Samuel, M. D. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (INDEX PAGE): | http://www.jwildlifedis.org/cgi/content/abstract/34/1/182 |
| Date Other: | Thu, 1 Jan 1998 00:00 -0600 |
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