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A cryopreservation method for Pasteurella multocida from wetland samples

Journal of Wildlife Diseases

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Abstract

A cryopreservation method and improved isolation techniques for detection of Pasteurella multocida from wetland samples were developed. Wetland water samples were collected in the field, diluted in dimethyl sulfoxide (DMSO, final concentration 10%), and frozen at -180 C in a liquid nitrogen vapor shipper. Frozen samples were transported to the laboratory where they were subsequently thawed and processed in Pasteurella multocida selective broth (PMSB) to isolate P. multocida. This method allowed for consistent isolation of 2 to 18 organisms/ml from water seeded with known concentrations of P. multocida. The method compared favorably with the standard mouse inoculation method and allowed for preservation of the samples until they could be processed in the laboratory.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A cryopreservation method for Pasteurella multocida from wetland samples
Series title:
Journal of Wildlife Diseases
Volume
34
Issue:
1
Year Published:
1998
Language:
English
Contributing office(s):
National Wildlife Health Center
Description:
p. 182-185
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
182
Last page:
185
Number of Pages:
4