Responses to in vitro and in vivo immunisations with Aeromonas salmonicida O antigen bacterins in rainbow trout (Oncorhynchus mykiss)
Links
- More information: Publisher Index Page (via DOI)
- Download citation as: RIS | Dublin Core
Abstract
Immunological assays were developed to test for the effects of immunisation with Aeromonas salmonicida O antigen bacterin in rainbow trout (Oncorhynchus mykiss) in vitro and in vivo. Trout spleen sections were immunised in vitro by incubation in media containing 100, 10, and 1 μg ml−1 of the A. salmonicida O antigen. After 10 days at 15°C, the individual sections were minced and cell suspensions assayed for the effects of the bacterin on non-specific and specific immune responses. Assays of the cell suspensions showed that the neutrophil oxidative activity, phagocytic capabilities, numbers of plaque-forming cells (PFC) and circulating antibody titres all increased in proportion to the bacterin dosage. Trout immunised in vivo with 100 μg by injection and by 100 μg ml−1 bath with the O antigen, showed similar responses to spleen sections immunised in vitro. The appearance of non-specific PFC against unlabelled sheep red blood cells was highest in spleens immunised in vitro and in the fish immunised by injection; non-specific PFC rarely occurred in O antigen bathed fish and in control fish. The development of these assays may enable biologists to follow the effects on the non-specific defence mechanisms and the specific immune response in cultivated fish populations after, immunisation with A. salmonicida bacterins and thus aid in the development of protective vaccination against furunculosis.
Publication type | Article |
---|---|
Publication Subtype | Journal Article |
Title | Responses to in vitro and in vivo immunisations with Aeromonas salmonicida O antigen bacterins in rainbow trout (Oncorhynchus mykiss) |
Series title | Fish and Shellfish Immunology |
DOI | 10.1016/S1050-4648(05)80064-6 |
Volume | 1 |
Issue | 4 |
Year Published | 1991 |
Language | English |
Publisher | Elsevier |
Contributing office(s) | Leetown Science Center |
Description | 10 p. |
First page | 251 |
Last page | 260 |
Google Analytic Metrics | Metrics page |