For many studies of the population dynamics, growth, and movement of juvenile Atlantic salmon Salmo salar, it is necessary to resample tagged individuals multiple times. However, common sampling techniques such as electrofishing can have negative effects on fish survival and growth, especially when individuals are repeatedly sampled. We describe an alternative to electrofishing that involves sampling at night with small, one-person seines. Juvenile Atlantic salmon in a small brook were tagged with passive integrated transponder tags and sampled 15 times by means of night seining, day electrofishing, and day seining techniques. Capturing juvenile Atlantic salmon by day seining was inefficient, resulting in a capture probability estimate of 0.18. The mean capture probability estimate was 0.45 during night seining samples and 0.78 during electrofishing samples. The total number of age-0 Atlantic salmon captured via night seining increased 4.5 times after fish attained a mean fork length of just over 60 mm. A much greater number of brook trout Salvelinus fontinalis and brown trout Salmo trutta were captured in electrofishing samples than in night seining samples. Night seining may prove useful when electrofishing is impractical, when threatened or endangered species exist, or when multiple recaptures of individuals are desired.