White-tailed kite (Elanus leucurus) populations in the 1930s were close to extirpation in the United States. But by the 1940s, an upward trend towards recovery was apparent and continued to their current stable population levels. These dramatic fluctuations in kite numbers may have been related to changes in rodent prey populations due to the conversion of native habitats to agriculture. To address this question, we evaluated the use of stable isotope analysis in determining if a shift in diet could be isotopically differentiated in current and historic kite populations. We first compared δ ¹³C, δ ¹⁵N, and δ ³⁴S values from present-day kite flight feathers and prey fur samples from four locations in California. The total ranges of isotope values for kite and their rodent prey were similar within each site. Carbon isotope values ranged from m 27.1 to m 22.2‰ in Arcata, m 26.1 to m 16.9‰ in Davis, m 27.0 to m 15.0‰ in Cosumnes, and m 28.2 to m 11.6‰ in Santa Barbara. Nitrogen isotope values ranged from 3.2 to 15.7‰ in Arcata, 2.8 to 12.7‰ in Davis, 4.0 to 15.7‰ in Cosumnes, and 1.7 to 20.0‰ in Santa Barbara. Sulfur isotope values ranged from m 7.8 to 12.4‰ in Arcata, m 1.1 to 9.2‰ in Davis, 0.7 to 10.9‰ in Cosumnes, and m 8.6 to 15.6‰ in Santa Barbara. Carbon, nitrogen, and sulfur isotope values at each site reflect typical trophic enrichments due to physiological processes. At each site, δ ¹³C and δ ¹⁵N values reflected the influence of a predominantly C₃ or a mixed C₃/C₄ plant community. Sulfur isotope values reflect the influence of predominant marine or terrestrial sulfur sources at each site. However, variability in isotope values may limit the usefulness of such analyses for addressing prey utilization and population dynamics.