| Abstract: | SYNOPSIS: Recent findings on the cryogenic preservation of semen from the crane, Grus canadensis pratensis and the domestic fowl, Gallus domesticus, are compared. Highest levels of post-thaw motility for crane semen (55%) were obtained when semen was diluted 1:1 with the Beltsville Poultry Semen Extender (BPSE) and held for 30 min at 5 C before it was equilibrated with 4% dimethyl sulfoxide (DMSO) for 15 min. In contrast, post-thaw motility for fowl spermatozoa was highest (80%) when semen was diluted 1:3 with BPSE and held for 60 min at 5 C before it was equilibrated with 4% DMSO for 60 min. Post-thaw motility of spermatozoa of both species was highest when the following freezing rates were used: l C per min from +5 to -20 C, 50 C per min from -20 to -80 C, then plunging into liquid nitrogen which resulted in a rate of 160 C per min from -80 to -196 C. One of four crane eggs resulting from insemination with frozen-thawed semen was fertile, whereas 27 of 55 fowl eggs were fertile, but this difference may have been due largely to fewer spermatozoa being inseminated into the female crane than into the fowl. |
| Genre: | Book chapter |
| ProdID: | 5210132 |
| Citation Author: | Sexton, T.J.; Gee, G.F. |
| Citation Contributing Office: | Patuxent Wildlife Research Center |
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| Citation Editor: | Watson, P.F. (editor) |
| Citation End Page: | 95 |
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| Citation Language: | English |
| Citation Larger Work Title: | Artificial Breeding of Non-Domestic Animals |
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| Citation Phsyical Description: | xxi, 376 |
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| Citation Public Comments: | OCLC no. 5676848. Symposium held at the Zoological Society of London on September 7-8, 1977. |
| Citation Publisher: | Academic Press |
| Citation Series: | Symposia of the Zoological Society of London |
| Citation Series Code: | |
| Citation Series Number: | 43 |
| Citation Search Results Text: | A comparative study on the cryogenic preservation of semen from the sandhill crane and the domestic fowl; 1978; Book chapter; 43; Artificial Breeding of Non-Domestic Animals; Sexton, T.J.; Gee, G.F. |
| Citation Start Page: | 89 |
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| Citation Year: | 1978 |
| Type: | citation/reference |
| Text: | A comparative study on the cryogenic preservation of semen from the sandhill crane and the domestic fowl; 1978; Book chapter; 43; Artificial Breeding of Non-Domestic Animals; Sexton, T.J.; Gee, G.F. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Tue, 9 Jun 2009 09:23 -0500 |
| Publisher: | Academic Press |
