Response of sago pondweed, a submerged aquatic macrophyte, to herbicides in three laboratory culture systems

Papers presented at the 2nd Symposium on Use of Plants for Toxicity Assessment, held in San Francisco, Calif., Apr. 23-24, 1990.
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Edited by:
Joseph W. Gorsuch, William R. Lower, Wun-cheng Wang, and M.A. Lewis


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The phytotoxicity of atrazine, paraquat, glyphosate, and alachlor to sago pondweed (Potamogeton pectinatus), a submerged aquatic macrophyte, was tested under three types of laboratory culture conditions. In each case, tests were conducted in static systems, the test period was four weeks, and herbicide exposure was chronic, resulting from a single addition of herbicide to the test vessels at the beginning of the test period. The three sets of test conditions employed were(1) axenic cultures in 125-mL flasks containing a nutrient media and sucrose; (2) a microcosm system employing 18.9-L buckets containing a sand, shell, and peat substrate; and (3) an algae-free system employing O.95-L jars containing reconstituted freshwater and a nutrient agar substrate. The primary variable measured was biomass production. Plants grew well in all three test systems, with biomass of untreated plants increasing by a factor of about 5 to 6.5 during the four-week test period. Biomass production in response to herbicide exposure differed significantly among culture systems, which demonstrates the need for a standardized testing protocol for evaluating the effects of toxics on submerged aquatic plants.

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Publication type:
Book chapter
Publication Subtype:
Book Chapter
Response of sago pondweed, a submerged aquatic macrophyte, to herbicides in three laboratory culture systems
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American Society for Testing and Materials
Publisher location:
Philadelphia, PA
Contributing office(s):
Patuxent Wildlife Research Center
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Larger Work Subtype:
Other Government Series
Larger Work Title:
Plants for Toxicity Assessment: Second Volume
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