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A prospective field evaluation of an enzyme immunoassay: Detection of eastern equine encephalomyelitis virus antigen in pools of Culiseta melanura

Journal of the American Mosquito Control Association

By:
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Abstract

A prospective field study was conducted to determine the sensitivity and specificity of an enzyme immunoassay (EIA) compared to virus isolation in cell culture for the detection of eastern equine encephalomyelitis (EEE) virus in naturally infected mosquitoes. A total of 10,811 adult female Culiseta melanura were collected in light traps during 1985 from four locations in Maryland. Eastern equine encephalomyelitis virus was isolated from 5 of 495 mosquito pools in African green monkey kidney and baby hamster kidney cell cultures. All five virus-infected pools were detected by the EIA, and all 490 uninfected pools were correctly scored as not containing virus. The EIA did not produce false positive or false negative results. Results support the assertion of previous researchers that the antigen detection EIA is a rapid, sensitive, specific, and simple alternative to traditional bioassays for the detection of EEE virus in mosquitoes.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A prospective field evaluation of an enzyme immunoassay: Detection of eastern equine encephalomyelitis virus antigen in pools of Culiseta melanura
Series title:
Journal of the American Mosquito Control Association
Volume
3
Issue:
3
Year Published:
1987
Language:
English
Contributing office(s):
Patuxent Wildlife Research Center
Description:
412-417
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
412
Last page:
417
Number of Pages:
6