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Combined extraction-cleanup column chromatographic procedure for determination of dicofol in avian eggs

Journal of the Association of Official Analytical Chemists

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Abstract

Dicofol in avian eggs was completely oxidized to dichlorobenzophenone (DCBP) when a hexane Soxhlet extraction procedure was used. This degradation did not occur with other avian tissues (muscle and liver). For this reason, a combined extraction-cleanup column chromatographic procedure, without added heat, was developed for the determination of dicofol in avian eggs. Homogenized subsamples of eggs were mixed with sodium sulfate, and the mixture was added as the top layer on a column prepacked with Florisil. The dicofol and other compounds of interest were then eluted with ethyl ether-hexane. The extracts, relatively free from lipids, were quantitated on a gas chromatograph equipped with a 63Ni electron-capture detector and a methyl silicone capillary column. Recoveries from chicken eggs, fortified with dicofol and other DDT-related compounds, averaged 96%. Analysis of eggs of eastern screech-owls, fed a meat diet containing 10 ppm technical Kelthane, showed that both dicofol and DCBP were present. Results were confirmed by gas chromatography/mass spectrometry. This method is rapid and reliable, involves a minimum of sample handling, and is well suited for high volume determination of dicofol in eggs and other avian tissues.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Combined extraction-cleanup column chromatographic procedure for determination of dicofol in avian eggs
Series title:
Journal of the Association of Official Analytical Chemists
Volume
71
Issue:
3
Year Published:
1988
Language:
English
Contributing office(s):
Patuxent Wildlife Research Center
Description:
539-542
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of the Association of Official Analytical Chemists
First page:
539
Last page:
542