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The characterization of axenic culture systems suitable for plant propagation and experimental studies of the submersed aquatic angiosperm Potamogeton pectinatus (Sago pondweed)

Estuaries

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Abstract

Clonal lines of the submersed aquatic angiosperm Potamogeton pectinatus were grown in three culture systems. The first, which used sucrose as a carbon source in a liquid medium, supported vigorous vegetative growth and can be used to propagate large numbers of plants in axenic conditions. In this culture system, plants were responsive to increasing photosynthetically active radiation (PAR) photon flux density (PFD) and were photosynthetically competent. However, their growth was heterotrophic and root development was poor. When these plants were transferred to a second nonaxenic culture system, which used 16-l buckets containing artificial sediments and tap water, growth was autotrophic and plants were morphologically identical to field-harvested P. pectinatus. The last culture system which consisted of a sand substrate and inorganic nutrient bathing solution aerated with 135 ml min-1 ambient air enhanced to 3.0% CO2 was axenic and supported autotrophic growth by plants that were also morphologically normal.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
The characterization of axenic culture systems suitable for plant propagation and experimental studies of the submersed aquatic angiosperm Potamogeton pectinatus (Sago pondweed)
Series title:
Estuaries
Volume
14
Issue:
1
Year Published:
1991
Language:
English
Contributing office(s):
Patuxent Wildlife Research Center
Description:
57-64
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
57
Last page:
64
Number of Pages:
8