Purification and immunochemical detections of ?-naphthoflavone- and phenobarbital-induced avian cytochrome P450 enzymes

Brown, R.L.; Levi, P.E.; Hodgson, E.; Melancon, M.J.

Abstract:

Livers from mallards (Anas platyrhynchos) were treated with either -naphthoflavone (50 mg/kg) or phenobarbital (70 mg/kg). Purification of induced hepatic cytochrome P450 was accomplished using both DEAE and hydroxyapatite columns, as well as sodium dodecyl sulfate polyacrylamide gel electrophoresis separation. Polyclonal antibodies to these proteins were then produced in young male New Zealand White rabbits. ?-naphthoflavone (?NF)- and phenobarbital(PB)-treated red-winged blackbird, screech owl, European starling and lesser scaup liver microsomes were analyzed in western blots for species cross-reactivity. Although all four of these avian species exhibited cross-reactivity with antibodies to ?NF-induced mallard P450, all but the lesser scaup revealed a protein of higher molecular weight than that of the ?NF-induced mallard. In addition, only the lesser scaup exhibited cross-reactivity with the anti-PB-induced mallard P450 antibodies.

Genre:

Article

ProdID:

5223200

Citation Author:

Brown, R.L.; Levi, P.E.; Hodgson, E.; Melancon, M.J.

Citation Contributing Office:

Patuxent Wildlife Research Center

Citation Datum:

Citation Day:

Citation Edition:

Citation Editor:

Citation End Page:

2298

Citation Issue:

Citation Keywords:

Citation Language:

English

Citation Larger Work Title:

Environmental Toxicology and Chemistry

Citation LatN:

Citation LatS:

Citation LonE:

Citation LonW:

Citation Month:

Citation No Pagination:

Citation Number Of Pages:

Citation Online Only Flag:

Citation Phsyical Description:

2293-2298

Citation Projection:

Citation Public Comments:

4892_Brown.pdf

Citation Publisher:

Citation Series:

Citation Series Code:

Citation Series Number:

Citation Search Results Text:

Purification and immunochemical detections of ?-naphthoflavone- and phenobarbital-induced avian cytochrome P450 enzymes; 1996; Article; Journal; Environmental Toxicology and Chemistry; Brown, R.L.; Levi, P.E.; Hodgson, E.; Melancon, M.J.