| Abstract: | We identified B-G-like genes in the whooping and Florida sandhill cranes and linked them to the major histocompatibility complex (MHC). We evaluated the inheritance of B-G-like genes in families of whooping and Florida sandhill cranes using restriction fragment patterns (RFPs). Two B-G-like genes, designated wcbgl and wcbg2, were located within 8 kb of one another. The fully sequenced wcbg2 gene encodes a B-G IgV-like domain, an additional Ig-like domain, a transmembrane domain, and a single heptad domain typical of ‘-helical coiled coils. Patterns of restriction fragments in DNA from the whooping crane and from a number of other species indicate that the B-G-like gene families of cranes are large with diverse sequences. Segregation of RFPs in families of Florida sandhill cranes provide evidence for genetic polymorphism in the B-G-like genes. The restriction fragments generally segregated in concert with MHC haplotypes assigned by serological typing and by single stranded conformational polymorphism (SSCP) assays based in the second exon of the crane MHC class I genes. This study supports the concept of a long-term association of polymorphic B-G-like genes with the MHC. It also establishes SSCP as a means for evaluating MHC genetic variability in cranes. |
| Genre: | Article |
| ProdID: | 5223333 |
| Citation Author: | Jarvi, S.I.; Goto, R.M.; Gee, G.F.; Briles, W.E.; Miller, M.M. |
| Citation Contributing Office: | Patuxent Wildlife Research Center |
| Citation Datum: | |
| Citation Day: | |
| Citation Edition: | |
| Citation Editor: | |
| Citation End Page: | 159 |
| Citation Issue: | 1 |
| Citation Keywords: | |
| Citation Language: | English |
| Citation Larger Work Title: | Journal of Heredity |
| Citation LatN: | |
| Citation LatS: | |
| Citation LonE: | |
| Citation LonW: | |
| Citation Month: | |
| Citation No Pagination: | |
| Citation Number Of Pages: | 8 |
| Citation Online Only Flag: | |
| Citation Phsyical Description: | 152-159 |
| Citation Projection: | |
| Citation Public Comments: | 5283_Jarvi.pdf |
| Citation Publisher: | |
| Citation Series: | |
| Citation Series Code: | |
| Citation Series Number: | |
| Citation Search Results Text: | Identification, inheritance, and linkage of B-G-like and MHC class I genes in cranes; 1999; Article; Journal; Journal of Heredity; Jarvi, S.I.; Goto, R.M.; Gee, G.F.; Briles, W.E.; Miller, M.M. |
| Citation Start Page: | 152 |
| Citation Volume: | 90 |
| Citation Year: | 1999 |
| Type: | citation/reference |
| Text: | Identification, inheritance, and linkage of B-G-like and MHC class I genes in cranes; 1999; Article; Journal; Journal of Heredity; Jarvi, S.I.; Goto, R.M.; Gee, G.F.; Briles, W.E.; Miller, M.M. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DOCUMENT): | http://jhered.oxfordjournals.org/cgi/reprint/90/1/152 |
| Date Other: | Wed, 16 Jun 2010 12:18 -0500 |
| Publisher: | |
