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Identification, inheritance, and linkage of B-G-like and MHC class I genes in cranes

Journal of Heredity

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Abstract

We identified B-G-like genes in the whooping and Florida sandhill cranes and linked them to the major histocompatibility complex (MHC). We evaluated the inheritance of B-G-like genes in families of whooping and Florida sandhill cranes using restriction fragment patterns (RFPs). Two B-G-like genes, designated wcbgl and wcbg2, were located within 8 kb of one another. The fully sequenced wcbg2 gene encodes a B-G IgV-like domain, an additional Ig-like domain, a transmembrane domain, and a single heptad domain typical of '-helical coiled coils. Patterns of restriction fragments in DNA from the whooping crane and from a number of other species indicate that the B-G-like gene families of cranes are large with diverse sequences. Segregation of RFPs in families of Florida sandhill cranes provide evidence for genetic polymorphism in the B-G-like genes. The restriction fragments generally segregated in concert with MHC haplotypes assigned by serological typing and by single stranded conformational polymorphism (SSCP) assays based in the second exon of the crane MHC class I genes. This study supports the concept of a long-term association of polymorphic B-G-like genes with the MHC. It also establishes SSCP as a means for evaluating MHC genetic variability in cranes.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Identification, inheritance, and linkage of B-G-like and MHC class I genes in cranes
Series title:
Journal of Heredity
Volume
90
Issue:
1
Year Published:
1999
Language:
English
Contributing office(s):
Patuxent Wildlife Research Center
Description:
152-159
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Heredity
First page:
152
Last page:
159
Number of Pages:
8