| Abstract: | In a continuation of our studies on the use of hepatic cytochromes P450 as a biomarker for contaminant exposure, BCNH eggs were collected from Baltimore Harbor (BH) (n = 20), Washington National Zoo (WNZ) (n = 13) and Chincoteague National Wildlife Refuge (CNWR) (reference location) (n = 20). Eggs were artificially incubated and sacrificed at pipping. Livers were snap frozen in liquid nitrogen and stored at -80?C until assay. Hepatic microsomes were prepared by differential centrifugation of homogenates and assayed for protein, benzyloxy-resorufin-O-dealkylase, (BROD) ethoxyresorufinO-dealkylase (EROD) and pentoxyresorufin-O-dealkylase (PROD). Monooxygenase assays were run in triplicate using a computer-coupled fluorometric microwell plate scanner. Values for EROD and BROD, but not PROD, from BH and WNZ were significantly greater (approximately double) than those from CNWR. Organochlorine pesticide residues were much higher in carcasses from BH and WNZ as compared to CNWR. Carcasses are presently being analyzed for PCB congeners. |
| Genre: | Article |
| ProdID: | 5224014 |
| Citation Author: | Melancon, M.J.; Rattner, B.A.; Rice, C.P.; Hines, R.K.; Eisemann, J. |
| Citation Contributing Office: | Patuxent Wildlife Research Center |
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| Citation Language: | English |
| Citation Larger Work Title: | Society of Environmental Toxicology and Chemistry, annual meeting abstract book |
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| Citation Phsyical Description: | 77 (abstract no. 357) |
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| Citation Search Results Text: | Hepatic microsomal monooxygenase activity in black-crowned night herons (BCNHS) from the Chesapeake basin; 1992; Article; Journal; Society of Environmental Toxicology and Chemistry, annual meeting abstract book; Melancon, M.J.; Rattner, B.A.; Rice, C.P.; Hines, R.K.; Eisemann, J. |
| Citation Start Page: | 77 (abstra |
| Citation Volume: | 13 |
| Citation Year: | 1992 |
| Type: | citation/reference |
| Text: | Hepatic microsomal monooxygenase activity in black-crowned night herons (BCNHS) from the Chesapeake basin; 1992; Article; Journal; Society of Environmental Toxicology and Chemistry, annual meeting abstract book; Melancon, M.J.; Rattner, B.A.; Rice, C.P.; Hines, R.K.; Eisemann, J. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Wed, 16 Jun 2010 12:18 -0500 |
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