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Hepatic microsomal monooxygenase activity in black-crowned night herons (BCNHS) from the Chesapeake basin

Society of Environmental Toxicology and Chemistry, annual meeting abstract book

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Abstract

In a continuation of our studies on the use of hepatic cytochromes P450 as a biomarker for contaminant exposure, BCNH eggs were collected from Baltimore Harbor (BH) (n = 20), Washington National Zoo (WNZ) (n = 13) and Chincoteague National Wildlife Refuge (CNWR) (reference location) (n = 20). Eggs were artificially incubated and sacrificed at pipping. Livers were snap frozen in liquid nitrogen and stored at -80?C until assay. Hepatic microsomes were prepared by differential centrifugation of homogenates and assayed for protein, benzyloxy-resorufin-O-dealkylase, (BROD) ethoxyresorufinO-dealkylase (EROD) and pentoxyresorufin-O-dealkylase (PROD). Monooxygenase assays were run in triplicate using a computer-coupled fluorometric microwell plate scanner. Values for EROD and BROD, but not PROD, from BH and WNZ were significantly greater (approximately double) than those from CNWR. Organochlorine pesticide residues were much higher in carcasses from BH and WNZ as compared to CNWR. Carcasses are presently being analyzed for PCB congeners.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Hepatic microsomal monooxygenase activity in black-crowned night herons (BCNHS) from the Chesapeake basin
Series title:
Society of Environmental Toxicology and Chemistry, annual meeting abstract book
Volume
13
Year Published:
1992
Language:
English
Contributing office(s):
Patuxent Wildlife Research Center
Description:
77 (abstract no. 357)
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
77 (abstra