| Abstract: | Livers from mink fed diets containing 0% (n = 12), 10% (n = 11), 20% (n = 12) and 40% (n = 10) SBC for 6 months contained 0.1, 2.2, 3.6, and 6.3 ug/g total PCBs, respectively. Hepatic microsomes were prepared and assayed for protein, arylhydrocarbon hydroxylase (AHH), benzyloxyresorufin-O-dealkylase (BROD), ethoxy-ROD (ER0D), pentoxy-ROD (PROD), and ethoxycoumarin-OD (ECOD). Mink fed SBC had increased AHH, EROD, and ECOD (group means 2.2-3.4 X control means), decreased BROD and unchanged PROD (the latter 2 assays indicators for phenobarbital-type induction in mammals). Three samples from each group were examined by western blot using a polyclonal anti-P450llB antibody and a monoclonal anti-P450lA antibody (MAb 1-12-3). Mink fed SBC showed induction of a protein recognized by anti-P450lA (8 X control), but had little protein recognized by anti-P450IlB. The monooxygenase activities and western blot data give a consistent picture of MC-type but not PB-type induction in mink fed SBC. |
| Genre: | Article |
| ProdID: | 5224016 |
| Citation Author: | Melancon, M.J.; LeCaptain, L.; Rattner, B.A.; Heaton, S.; Aulerich, R.; Tillitt, D.; Stegeman, J.J.; Woodin, B. |
| Citation Contributing Office: | Patuxent Wildlife Research Center |
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| Citation Language: | English |
| Citation Larger Work Title: | Society of Environmental Toxicology and Chemistry, annual meeting abstract book |
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| Citation Phsyical Description: | 197 (abstract no. TA6F23) |
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| Citation Search Results Text: | Hepatic microsomal cytochromes P450 in mink fed Saginaw Bay carp (SBC); 1992; Article; Journal; Society of Environmental Toxicology and Chemistry, annual meeting abstract book; Melancon, M.J.; LeCaptain, L.; Rattner, B.A.; Heaton, S.; Aulerich, R.; Tillitt, D.; Stegeman, J.J.; Woodin, B. |
| Citation Start Page: | 197 (abstr |
| Citation Volume: | 13 |
| Citation Year: | 1992 |
| Type: | citation/reference |
| Text: | Hepatic microsomal cytochromes P450 in mink fed Saginaw Bay carp (SBC); 1992; Article; Journal; Society of Environmental Toxicology and Chemistry, annual meeting abstract book; Melancon, M.J.; LeCaptain, L.; Rattner, B.A.; Heaton, S.; Aulerich, R.; Tillitt, D.; Stegeman, J.J.; Woodin, B. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Wed, 16 Jun 2010 12:18 -0500 |
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