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Oxidative stress induced in PCB 126-exposed northern leopard frogs, Rana pipiens

Journal of Toxicology and Environmental Health, Part A

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DOI: 10.1080/15287390600974676

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Abstract

Northern leopard frogs Rana pipiens exposed to PCB 126 (3,3',4,4',5-pentachlorobiphenyl) were examined for hepatic oxidative stress. In a dose-response study, northern leopard frogs were injected intraperitoneally with either PCB 126 in corn oil (0.2, 0.7, 2.3, or 7.8 mg/kg body weight) or corn oil alone. In a time-course study, frogs received 7.8 mg/kg or corn oil alone, and were examined at 1, 2, 3, and 4 wk after dosing. Hepatic concentrations of reduced glutathione (GSH), thiobarbituric acid-reactive substances (TBARS), and total sulfhydryls (total SH), as well as activities of glutathione peroxidase (GSH-P), GSSG reductase (GSSG-R), glucose-6-phosphate dehydrogenase (G-6-PDH), and glutathione S-transferase (GSH-S-T) were measured. In the dose-response experiment, few effects were apparent 1 wk after dosing. In the time-course experiment, significant changes were observed in the 7.8-mg/kg group at 2 wk or more posttreatment. Hepatic concentrations of GSH and TBARS were higher than in corresponding controls at wk 3 and 4; the activities of GSSG-R and GSH-S-T were higher than in controls at wk 2 and 4; and the activity of G-6-PDH was increased at wk 2 and 4. These data collectively indicate that altered glutathione metabolism and oxidative stress occurred and were indicative of both toxicity and induction of protective mechanisms in frogs exposed to PCB. A similar delay in response was reported in fish and may relate to lower metabolic rate and physiological reactions in ectothermic vertebrates

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Oxidative stress induced in PCB 126-exposed northern leopard frogs, Rana pipiens
Series title:
Journal of Toxicology and Environmental Health, Part A
DOI:
10.1080/15287390600974676
Volume
70
Issue:
8
Year Published:
2007
Language:
English
Contributing office(s):
Patuxent Wildlife Research Center
Description:
676-681
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Toxicology and Environmental Health, Part A
First page:
676
Last page:
681
Number of Pages:
6