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Liquid chromatographic method for determining the concentration of bisazir in water

Journal of AOAC International

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Abstract

Barrier dams, traps, and lampricides are the techniques currently used by the Great Lakes Fishery Commission to control sea lampreys (Petromyzon marinus) in the Great Lakes. To augment these control techniques, a sterile-male-release research program was initiated at the Lake Huron Biological Station. Male sea lampreys were sterilized by intraperitoneal injection of the chemical sterilant P,P-bis(1-aziridinyl)-N-methylphosphinothioic amide (bisazir). An analytical method was needed to quantitate the concentration of bisazir in water and to routinely verify that bisazir (>25 μg/L) does not persist in the treated effluent discharged from the sterilization facility to Lake Huron. A rapid, accurate, and sensitive liquid chromatographic (LC) method was developed for determining bisazir in water. Bisazir was dissolved in Lake Huron water; extracted and concentrated on a C18 solid-phase extraction column; eluted with methanol; and quantitated by reversed-phase LC using a C18 column, a mobile phase of 70% water and 30% methanol (v/v), and UV detection (205 nm). Bisazir retention time was 7-8 min; total run time was about 20 min. Method detection limit for bisazir dissolved in Lake Huron water was about 15 μg/L. Recovery from Lake Huron water fortified with bisazir at 100 μg/L was 94% (95% confidence interval, 90.2-98.2%).

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Liquid chromatographic method for determining the concentration of bisazir in water
Series title:
Journal of AOAC International
Volume
80
Issue:
5
Year Published:
1997
Language:
English
Publisher:
AOAC International
Publisher location:
Gaithersburg, MD
Contributing office(s):
Great Lakes Science Center
Description:
6 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
1111
Last page:
1116
Number of Pages:
5