Liquid chromatographic method for determining the concentration of bisazir in water
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Abstract
Barrier dams, traps, and lampricides are the techniques currently used by the Great Lakes Fishery Commission to control sea lampreys {Petromyzon marinug) in the Great Lakes. To augment these control techniques, a sterile-male-release research program was initiated at the Lake Huron Biological Station. Male sea lampreys were sterilized by intraperitoneal injection of the chemical sterilant P,P-bis(1-aziridinyl)-Nmethylphosphinothioic amide (bisazir). An analytical method was needed to quantitate the concentration of bisazir in water and to routinely verify that bisazir (>25 μg/L) does not persist in the treated effluent discharged from the sterilization facility to Lake Huron. A rapid, accurate, and sensitive liquid chromatographic (LC) method was developed for determining bisazir in water. Bisazir was dissolved in Lake Huron water; extracted and concentrated on a C18 solid-phase extraction column; eluted with methanol; and quantitated by reversed-phase LC using a Cis column, amobile phase of 70% water and 30% methanol (v/v), and UV detection (205 nm). Bisazir retention time was 7-8 min; total run time was about 20 min. Method detection limit for bisazir dissolved in Lake Huron water was about 15 μg/L. Recovery from Lake Huron water fortified with bisazir at 100 μg/L was 94% (95% confidence interval, 90.2-98.2%).
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Liquid chromatographic method for determining the concentration of bisazir in water |
| Series title | Journal of AOAC International |
| DOI | 10.1093/jaoac/80.5.1111 |
| Volume | 80 |
| Issue | 5 |
| Year Published | 1997 |
| Language | English |
| Publisher | Oxford Academic |
| Contributing office(s) | Great Lakes Science Center |
| Description | 6 p. |
| First page | 1111 |
| Last page | 1116 |
| Google Analytic Metrics | Metrics page |