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Allantoinase in lake trout (Salvelinus namaycush): in vitro effects of PCBs, DDT and metals

Comparative Biochemistry and Physiology Part C

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DOI: 10.1016/0306-4492(79)90102-3

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Abstract

1. Allantoinase, an enzyme in the purine-urea cycle, was found in livers of Salvelinus namaycush (Osteichthyes: Salmoniformes). 2. The enzyme was active from pH 6.6 to 8.2 at 37°C and from pH 7.4 to 9.0 at 10°C and had an Arrhenius energy of activation of 11.0 kcal/mol and a temperature quotient of 2.0. The Km of the enzyme homogenate was 8.4 mM allantoin. 3. The concentration of inorganic metals at which 50% inhibition occurred during in vitro exposure were 6.0 mg/l Cu2+, 6.7 mg/l Cd2+, 34 mg/l Hg2+ and 52 mg/l Pb2+. The in vitro sensitivity to PCBs, DDT and DDE and kinetics in the presence of metals were determined. 4. Allantoinase activity was negatively correlated with body length for fish from Lake Michigan but not from Lake Superior or the laboratory.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Allantoinase in lake trout (Salvelinus namaycush): in vitro effects of PCBs, DDT and metals
Series title:
Comparative Biochemistry and Physiology Part C
DOI:
10.1016/0306-4492(79)90102-3
Volume
62
Issue:
1
Year Published:
1979
Language:
English
Publisher:
Elsevier
Publisher location:
Amsterdam, Netherlands
Contributing office(s):
Great Lakes Science Center
Description:
5 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Comparative Biochemistry and Physiology Part C
First page:
71
Last page:
75