| Abstract: | We used 363 blood samples collected from wild canvasback ducks (Aythya valisineria) at Catahoula Lake, Louisiana, U.S.A. to evaluate the effect of sample storage time on the efficacy of erythrocytic protoporphyrin as an indicator of lead exposure. The protoporphyrin concentration of each sample was determined by hematofluorometry within 5 min of blood collection and after refrigeration at 4 °C for 24 and 48 h. All samples were analyzed for lead by atomic absorption spectrophotometry. Based on a blood lead concentration of ≥0.2 ppm wet weight as positive evidence for lead exposure, the protoporphyrin technique resulted in overall error rates of 29%, 20%, and 19% and false negative error rates of 47%, 29% and 25% when hematofluorometric determinations were made on blood at 5 min, 24 h, and 48 h, respectively. False positive error rates were less than 10% for all three measurement times. The accuracy of the 24-h erythrocytic protoporphyrin classification of blood samples as positive or negative for lead exposure was significantly greater than the 5-min classification, but no improvement in accuracy was gained when samples were tested at 48 h. The false negative errors were probably due, at least in part, to the lag time between lead exposure and the increase of blood protoporphyrin concentrations. False negatives resulted in an underestimation of the true number of canvasbacks exposed to lead, indicating that hematofluorometry provides a conservative estimate of lead exposure. |
| Genre: | Article |
| ProdID: | 70006598 |
| Citation Author: | Franson, J. C.; Hohman, W. L.; Moore, J. L.; Smith, M. R. |
| Citation Contributing Office: | National Wildlife Health Center |
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| Citation End Page: | 188 |
| Citation Issue: | 2 |
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| Citation Language: | English |
| Citation Larger Work Title: | Environmental Monitoring and Assessment |
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| Citation Number Of Pages: | 8 |
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| Citation Phsyical Description: | 8 p. |
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| Citation Public Comments: | Abstract has subscript/superscript to be fixed |
| Citation Publisher: | Springer |
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| Citation Search Results Text: | The efficacy of protoporphyrin as a predictive biomarker for lead exposure in canvasback ducks: effect of sample storage time; 1996; Article; Journal; Environmental Monitoring and Assessment; Franson, J. C.; Hohman, W. L.; Moore, J. L.; Smith, M. R. |
| Citation Start Page: | 181 |
| Citation Volume: | 43 |
| Citation Year: | 1996 |
| Type: | citation/reference |
| Text: | The efficacy of protoporphyrin as a predictive biomarker for lead exposure in canvasback ducks: effect of sample storage time; 1996; Article; Journal; Environmental Monitoring and Assessment; Franson, J. C.; Hohman, W. L.; Moore, J. L.; Smith, M. R. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1007/BF00398606 |
| Date Other: | Sun, 1 Jan 2012 14:04 -0600 |
| Publisher: | Springer |
