thumbnail

A new selective enrichment procedure for isolating Pasteurella multocida from avian and environmental samples

Avian Diseases

Abstract has subscript/superscript to be fixed
By:
, , and

Links

Abstract

A selective enrichment procedure, using two new selective media, was developed to isolate Pasteurella multocida from wild birds and environmental samples. These media were developed by testing 15 selective agents with six isolates of P. multocida from wild avian origin and seven other bacteria representing genera frequently found in environmental and avian samples. The resulting media—Pasteurella multocida selective enrichment broth and Pasteurella multocida selective agar—consisted of a blood agar medium at pH 10 containing gentamicin, potassium tellurite, and amphotericin B. Media were tested to determine: 1) selectivity when attempting isolation from pond water and avian carcasses, 2) sensitivity for detection of low numbers of P. multocida from pure and mixed cultures, 3) host range specificity of the media, and 4) performance compared with standard blood agar. With the new selective enrichment procedure, P. multocida was isolated from inoculated (60 organisms/ml) pond water 84% of the time, whereas when standard blood agar was used, the recovery rate was 0%.

Geospatial Extents

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A new selective enrichment procedure for isolating Pasteurella multocida from avian and environmental samples
Series title:
Avian Diseases
Volume
38
Issue:
2
Year Published:
1994
Language:
English
Publisher:
American Association of Avian Pathologists
Publisher location:
Jacksonville, FL
Contributing office(s):
National Wildlife Health Center
Description:
7 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Avian Diseases
First page:
317
Last page:
324
Country:
United States
State:
California, Illinois, Indiana, Iowa, Maryland, Massachusetts, Minnesota, Mississippi, Nebraska, New Mexico, New York, South Carolina, Texas, Virginia, West Virginia, Wisconsin, Wyoming
Online Only (Y/N):
N
Additional Online Files(Y/N):
N