| Abstract: | Inclusion body disease of cranes (IBDC) herpesvirus kills some infected cranes and persists in convalescent animals. To enable further study and rapid identification of carrier animals, we developed a monoclonal antibody (MAb) to IBDC virus and used it in immunohistochemistry and a competitive enzyme-linked immunosorbent assay (ELISA). We used conventional techniques to make murine MAbs directed against IBDC virus purified from infected duck embryo cells. Hybridomas reacting in an ELISA with IBDC virus but not uninfected duck embryo cells were characterized by radioimmunoprecipitation, in situ immunohistochemistry, and competitive ELISA with neutralizing and nonneutralizing crane sera. MAb 2C11 immunoprecipitated 59-, 61-, and 110-kD proteins from IBDC virus-infected but not uninfected cells and stained glutaraldehyde-fixed IBDC virus plaques but not surrounding uninfected duck embryo cells in vitro. Antibody 2C11 did not react with duck embryo cells infected with falcon herpesvirus, psittacine herpesvirus, infectious laryngotracheitis, pigeon herpesvirus, or duck plague virus. A competitive ELISA using antibody 2C11 identified most sera that were positive in the neutralization test. This antibody will be useful in further characterizing IBDC virus, its pathogenesis, and its natural history. |
| Genre: | Article |
| ProdID: | 70006637 |
| Citation Author: | Letchworth, G. J.; Fishel, J. R.; Hansen, W. R. |
| Citation Contributing Office: | National Wildlife Health Center |
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| Citation End Page: | 816 |
| Citation Issue: | 4 |
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| Citation Language: | English |
| Citation Larger Work Title: | Avian Diseases |
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| Citation Number Of Pages: | 9 |
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| Citation Phsyical Description: | 9 p. |
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| Citation Public Comments: | Abstract has subscript/superscript to be fixed |
| Citation Publisher: | American Association of Avian Pathologists |
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| Citation Search Results Text: | A monoclonal antibody to inclusion body disease of cranes virus enabling specific immunohistochemistry and competitive ELISA; 1997; Article; Journal; Avian Diseases; Letchworth, G. J.; Fishel, J. R.; Hansen, W. R. |
| Citation Start Page: | 808 |
| Citation Volume: | 41 |
| Citation Year: | 1997 |
| Type: | citation/reference |
| Text: | A monoclonal antibody to inclusion body disease of cranes virus enabling specific immunohistochemistry and competitive ELISA; 1997; Article; Journal; Avian Diseases; Letchworth, G. J.; Fishel, J. R.; Hansen, W. R. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (INDEX PAGE): | http://www.jstor.org/stable/1592333 |
| Date Other: | Sun, 1 Jan 2012 10:18 -0600 |
| Publisher: | American Association of Avian Pathologists |
