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Biological characterisation of Sarcocystis neurona isolated from a Southern sea otter (Enhydra lutris nereis)

International Journal for Parasitology

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DOI: 10.1016/S0020-7519(00)00034-5

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Abstract

Sarcocystis neurona was isolated from the brain of a juvenile, male southern sea otter (Enhydra lutris nereis) suffering from CNS disease. Schizonts and merozoites in tissue sections of the otter's brain reacted with anti-S. neurona antiserum immunohistochemically. Development in cell culture was by endopolyogeny and mature schizonts were first observed at 3 days postinoculation. PCR of merozoite DNA using primer pairs JNB33/JNB54 and restriction enzyme digestion of the 1100 bp product with Dra I indicated the organism was S. neurona. Four of four interferon-γ gene knockout mice inoculated with merozoites developed S. neurona-associated encephalitis. Antibodies to S. neurona but not Sarcocystis falcatula, Toxoplasma gondii, or Neospora caninum were present in the serum of inoculated mice. This is the first isolation of S. neurona from the brain of a non-equine host.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Biological characterisation of Sarcocystis neurona isolated from a Southern sea otter (Enhydra lutris nereis)
Series title:
International Journal for Parasitology
DOI:
10.1016/S0020-7519(00)00034-5
Volume
30
Issue:
5
Year Published:
2000
Language:
English
Publisher:
Elsevier
Publisher location:
Amsterdam, Netherlands
Contributing office(s):
National Wildlife Health Center
Description:
8 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
International Journal for Parasitology
First page:
617
Last page:
624
Number of Pages:
8