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Post-column reaction for simultaneous analysis of chromatic and leuco forms of malachite green and crystal violet by high-performance liquid chromatography with photometric detection

Journal of Chromatography

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DOI: 10.1016/S0021-9673(01)89252-9

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Abstract

The chromatic and leuco forms of malachite green and crystal violet were readily separated and detected by a sensitive and selective high-performance liquid chromatographic procedure. The chromatic and leuco forms of the dyes were separated within 11 min on a C18 column with a mobile phase of 0.05 M sodium acetate and 0.05 M acetic acid in water (19%) and methanol (81%). A reaction chamber, containing 10% PbO2 in Celite 545, was placed between the column and the spectrophotometric detector to oxidize the leuco forms of the dyes to their chromatic forms. Chromatic and leuco malachite green were quantified by their absorbance at 618 nm; and chromatic and leuco Crystal Violet by their absorbance at 588 nm. Detection limits for chromatic and leuco forms of both dyes ranged from 0.12 to 0.28 ng. A linear range of 1 to 100 ng was established for both forms of the dyes.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Post-column reaction for simultaneous analysis of chromatic and leuco forms of malachite green and crystal violet by high-performance liquid chromatography with photometric detection
Series title:
Journal of Chromatography
DOI:
10.1016/S0021-9673(01)89252-9
Volume
536
Issue:
1-2
Year Published:
1991
Language:
English
Publisher:
Elsevier
Publisher location:
Amsterdam, Netherlands
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
6 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
217
Last page:
222
Number of Pages:
6