| Abstract: | N-sodium-N-chloro-rho-toluenesulfonamide (chloramine-T) effectively controls bacterial gill disease (BGD) in cultured fishes, BGD, a common disease of hatchery-reared salmonids, causes more fish losses than any other disease among these species, This study describes a liquid chromatographic (LC) method that is capable of direct, simultaneous analysis of chloramine-T and its primary degradation product, rho-toluenesulfonamide (rho-TSA), in water. The procedure involves reversed-phase (C-18) LC analysis with ion suppression, using 0.01 M phosphate buffer at pH 3. The mobile phase is phosphate buffer-acetonitrile (60 + 40) at 1 mL/min. Both chemicals can be detected with a UV spectrophotometer at 229 nm; the method is linear up to 40 mg, chloramine-T or rho-TSA/L. Mean recoveries were 96.4 +/- 6.1% for water samples fortified with 0.03 mg chloramine-T/L and 95.3 +/- 4.6% for water samples fortified with 0.005 mg rho-TSA/L. Limits of detection without sample enrichment for chloramine-T and rho-TSA are 0.01 mg/L and 0.001 mg/L, respectively. |
| Genre: | Article |
| ProdID: | 70006705 |
| Citation Author: | Hoffman, G. L. |
| Citation Contributing Office: | Leetown Science Center |
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| Citation End Page: | 3 |
| Citation Issue: | 4 |
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| Citation Language: | English |
| Citation Larger Work Title: | Fish Health Section Newsletter |
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| Citation Phsyical Description: | 1 p. |
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| Citation Publisher: | American Fisheries Society |
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| Citation Search Results Text: | Is nothing sacred?; 1980; Article; Journal; Fish Health Section Newsletter; Hoffman, G. L. |
| Citation Start Page: | 3 |
| Citation Volume: | 8 |
| Citation Year: | 1980 |
| Type: | citation/reference |
| Text: | Is nothing sacred?; 1980; Article; Journal; Fish Health Section Newsletter; Hoffman, G. L. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Sun, 1 Jan 2012 00:00 -0600 |
| Publisher: | American Fisheries Society |
