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A rapid high-performance liquid-chromatographic method for simultaneously determining the concentrations of TFM and Bayer 73 in water during lampricide treatments

Canadian Journal of Fisheries and Aquatic Sciences

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DOI: 10.1139/f82-105

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Abstract

The high-performance liquid-chromatography (HPLC) procedure requires only minutes per sample, is specific, and is relatively sensitive (limit of detection < 0.005 mg/L for both chemicals). A combined buffer (pH 4) and internal standard (3-chlorodiphenylamine) reagent is added to the water sample, which is injected through a Sep Pak C18 disposable cartridge. The cartridge adsorbs and retains both the lampricides and the internal standard. The quantitative elution of the three chemicals from the cartridge with a small volume of methanol effectively concentrates the sample and provides sample cleanup. The methanol extract is then analyzed directly by HPLC on an MCH 10 reverse phase column by using a methanol:0.01 mol/L acetate buffer (87:13, v:v) as the mobile phase at 2 mL/min and detected by ultraviolet spectrophotometry at 330 (or 254) nm. A microprocessor data system further facilitates the procedure by quantifying off-scale peaks and yielding results directly in units of concentration (mg/L).

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A rapid high-performance liquid-chromatographic method for simultaneously determining the concentrations of TFM and Bayer 73 in water during lampricide treatments
Series title:
Canadian Journal of Fisheries and Aquatic Sciences
DOI:
10.1139/f82-105
Volume
39
Issue:
5
Year Published:
1982
Language:
English
Publisher:
NRC Research Press
Publisher location:
Ottawa, Ontario
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
5 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Canadian Journal of Fisheries and Aquatic Sciences
First page:
778
Last page:
782