| Abstract: | The high-performance liquid-chromatography (HPLC) procedure requires only minutes per sample, is specific, and is relatively sensitive (limit of detection < 0.005 mg/L for both chemicals). A combined buffer (pH 4) and internal standard (3-chlorodiphenylamine) reagent is added to the water sample, which is injected through a Sep Pak C18 disposable cartridge. The cartridge adsorbs and retains both the lampricides and the internal standard. The quantitative elution of the three chemicals from the cartridge with a small volume of methanol effectively concentrates the sample and provides sample cleanup. The methanol extract is then analyzed directly by HPLC on an MCH 10 reverse phase column by using a methanol:0.01 mol/L acetate buffer (87:13, v:v) as the mobile phase at 2 mL/min and detected by ultraviolet spectrophotometry at 330 (or 254) nm. A microprocessor data system further facilitates the procedure by quantifying off-scale peaks and yielding results directly in units of concentration (mg/L). |
| Genre: | Article |
| ProdID: | 70006788 |
| Citation Author: | Dawson, V. K. |
| Citation Contributing Office: | Upper Midwest Environmental Sciences Center |
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| Citation End Page: | 782 |
| Citation Issue: | 5 |
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| Citation Language: | English |
| Citation Larger Work Title: | Canadian Journal of Fisheries and Aquatic Sciences |
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| Citation Number Of Pages: | 5 |
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| Citation Phsyical Description: | 5 p. |
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| Citation Public Comments: | None |
| Citation Publisher: | NRC Research Press |
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| Citation Search Results Text: | A rapid high-performance liquid-chromatographic method for simultaneously determining the concentrations of TFM and Bayer 73 in water during lampricide treatments; 1982; Article; Journal; Canadian Journal of Fisheries and Aquatic Sciences; Dawson, V. K. |
| Citation Start Page: | 778 |
| Citation Volume: | 39 |
| Citation Year: | 1982 |
| Type: | citation/reference |
| Text: | A rapid high-performance liquid-chromatographic method for simultaneously determining the concentrations of TFM and Bayer 73 in water during lampricide treatments; 1982; Article; Journal; Canadian Journal of Fisheries and Aquatic Sciences; Dawson, V. K. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1139/f82-105 |
| Date Other: | Sun, 1 Jan 2012 19:55 -0600 |
| Publisher: | NRC Research Press |
