| Abstract: | The fate of a single i.v. dose (120 μg/kg) of the piscicide [14C]rotenone was evaluated in rainbow trout for periods up to 72 h after dosing. Rotenone was rapidly cleared from the plasma; less than 2% of the dose remained in the plasma compartment after 20 min. The highest concentrations of rotenone residues (% dose/g tissue) were in the hepatobiliary system, bile, intestine, and in heart, lateral line swimming muscle, and posterior kidney; tissues that are highly dependent on oxidative metabolism. Although rotenone activity was present in all cell fractions examined, greater than 40% was associated with the mitochondrial fraction of liver, kidney, and muscle. More than 85% of the activity extracted from these tissues, except the liver, was parent rotenone. Elimination from whole body and major tissue depots conformed to simple first-order kinetics; the estimated half-life from whole body was 68.5 h. Branchial elimination accounted for 5% of the injected dose over a 4-h period, and urinary elimination was less than 2% over a 48-h period. Rotenone was eliminated essentially unchanged across the gills; however, parent rotenone was not found in either urine or bile. More than 80% of the activity in both urine and bile eluted from HPLC chromatographs as a highly polar fraction that was not hydrolyzed by incubation with either β-glucuronidase or sulfatase. The results imply that hepatobiliary excretion is the major route of elimination for rotenone residues in the trout and that metabolism to a more polar form is a prerequisite for elimination in both the bile and the urine |
| Genre: | Article |
| ProdID: | 70006833 |
| Citation Author: | Gingerich, W. H. |
| Citation Contributing Office: | Upper Midwest Environmental Sciences Center |
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| Citation End Page: | 40 |
| Citation Issue: | 1 |
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| Citation Language: | English |
| Citation Larger Work Title: | Aquatic Toxicology |
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| Citation Number Of Pages: | 14 |
| Citation Online Only Flag: | |
| Citation Phsyical Description: | 13 p. |
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| Citation Public Comments: | Abstract has subscript/superscript to be fixed |
| Citation Publisher: | Elsevier |
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| Citation Search Results Text: | Tissue distribution and elimination of rotenone in rainbow trout; 1986; Article; Journal; Aquatic Toxicology; Gingerich, W. H. |
| Citation Start Page: | 27 |
| Citation Volume: | 8 |
| Citation Year: | 1986 |
| Type: | citation/reference |
| Text: | Tissue distribution and elimination of rotenone in rainbow trout; 1986; Article; Journal; Aquatic Toxicology; Gingerich, W. H. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1016/0166-445X(86)90070-6 |
| Date Other: | Wed, 1 Jan 1986 11:04 -0600 |
| Publisher: | Elsevier |
