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Tissue distribution and elimination of rotenone in rainbow trout

Aquatic Toxicology

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DOI: 10.1016/0166-445X(86)90070-6

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Abstract

The fate of a single i.v. dose (120 μg/kg) of the piscicide [14C]rotenone was evaluated in rainbow trout for periods up to 72 h after dosing. Rotenone was rapidly cleared from the plasma; less than 2% of the dose remained in the plasma compartment after 20 min. The highest concentrations of rotenone residues (% dose/g tissue) were in the hepatobiliary system, bile, intestine, and in heart, lateral line swimming muscle, and posterior kidney; tissues that are highly dependent on oxidative metabolism. Although rotenone activity was present in all cell fractions examined, greater than 40% was associated with the mitochondrial fraction of liver, kidney, and muscle. More than 85% of the activity extracted from these tissues, except the liver, was parent rotenone. Elimination from whole body and major tissue depots conformed to simple first-order kinetics; the estimated half-life from whole body was 68.5 h. Branchial elimination accounted for 5% of the injected dose over a 4-h period, and urinary elimination was less than 2% over a 48-h period. Rotenone was eliminated essentially unchanged across the gills; however, parent rotenone was not found in either urine or bile. More than 80% of the activity in both urine and bile eluted from HPLC chromatographs as a highly polar fraction that was not hydrolyzed by incubation with either β-glucuronidase or sulfatase. The results imply that hepatobiliary excretion is the major route of elimination for rotenone residues in the trout and that metabolism to a more polar form is a prerequisite for elimination in both the bile and the urine

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Tissue distribution and elimination of rotenone in rainbow trout
Series title:
Aquatic Toxicology
DOI:
10.1016/0166-445X(86)90070-6
Volume
8
Issue:
1
Year Published:
1986
Language:
English
Publisher:
Elsevier
Publisher location:
Amsterdam, Netherlands
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
13 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Aquatic Toxicology
First page:
27
Last page:
40