| Abstract: | The distribution and loss of radioactivity from tissues were determined in 60 juvenile channel catfish (Ictalurus punctatus) following oral dosing with the candidate fish therapeutant Sarafin® ([14C] sarafloxacin hydrochloride) at 10 mg/kg for 5 consecutive days. Twelve groups of 5 fish each were sampled at selected times ranging from 3 to 240 h after the last dose was administered, The concentration and content of sarafloxacin-equivalent activity was determined in liver, gallbladder, kidney, skin, and skinless fillet by sample oxidation and liquid scintillation counting; content of sarafloxacin-equivalent activity was determined in stomach and anterior and posterior intestines, Skinless fillet tissues were also analyzed for sarafloxacin and for potential metabolites by gradient-elution high-performance liquid chromatography (HPLC) with in-line radiometric and fluorescence detection, Loss of radioactivity from the whole body conformed to a bimodal elimination pattern with a rapid initial phase (t1/2=11 h) and a slower secondary phase (t1/2=222 h). Tissue and contents of the gastrointestinal tract (i.e. stomach and anterior and posterior intestines) were a principal depot of activity during the first four sample times (3, 6, 12, and 24 h); the combined head, skeleton, and fins (i.e. residual carcass) were the principal depot of activity in samples taken after 24 h. Of those tissues sampled 3 h after the last dose, relative sarafloxacin concentration was greatest in the liver (4.06 μg equivalents/g) and least in the residual carcass (1.13 μg equivalents/g), Intermediate concentrations were found in the kidney (2.04 μg equivalents/g), skinless fillet (1.71 μg equivalents/ g), and the skin (1.51 μg equivalents/g). Concentrations of sarafloxacin-equivalent residues in edible skinless fillet were consistently among the lowest of all tissues examined. The highest mean concentration of parent-equivalent material in the fillet tissue was found 12 h after administration of the last dose (2.27 μg equivalents/g) and declined thereafter, Sarafloxacin constituted between 80 and 90% of the extractable radioactive residues from the fillet homogenates. No other peaks were resolved in any of the fillet tissue samples analyzed by HPLC with in-line radiometric detection. |
| Genre: | Article |
| ProdID: | 70006839 |
| Citation Author: | Gingerich, W. H.; Meinertz, J. R.; Dawson, V. K.; Gofus, J. E.; Delaney, L. J.; Bunnell, P. R. |
| Citation Contributing Office: | Upper Midwest Environmental Sciences Center |
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| Citation End Page: | 36 |
| Citation Issue: | 1-2 |
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| Citation Language: | English |
| Citation Larger Work Title: | Aquaculture |
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| Citation Number Of Pages: | 14 |
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| Citation Phsyical Description: | 14 p. |
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| Citation Public Comments: | Abstract has subscript/superscript to be fixed |
| Citation Publisher: | Elsevier |
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| Citation Search Results Text: | Distribution and elimination of [14C] sarafloxacin hydrochloride from tissues of juvenile channel catfish (Ictalurus punctatus); 1995; Article; Journal; Aquaculture; Gingerich, W. H.; Meinertz, J. R.; Dawson, V. K.; Gofus, J. E.; Delaney, L. J.; Bunnell, P. R. |
| Citation Start Page: | 23 |
| Citation Volume: | 131 |
| Citation Year: | 1995 |
| Type: | citation/reference |
| Text: | Distribution and elimination of [14C] sarafloxacin hydrochloride from tissues of juvenile channel catfish (Ictalurus punctatus); 1995; Article; Journal; Aquaculture; Gingerich, W. H.; Meinertz, J. R.; Dawson, V. K.; Gofus, J. E.; Delaney, L. J.; Bunnell, P. R. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1016/0044-8486(94)00362-R |
| Date Other: | Sun, 1 Jan 2012 10:45 -0600 |
| Publisher: | Elsevier |
