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Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry

Analytical Chemistry

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Abstract

A mixture of closely related streptomyces fermentation products, antimycin A, Is separated, and the components are identified by using reversed-phase high-performance liquid chromatography with directly linked 400-MHz proton nuclear magnetic resonance detection. Analyses of mixtures of three amino acids, alanine, glycine, and valine, are used to determine optimal measurement conditions. Sensitivity increases of as much as a factor of 3 are achieved, at the expense of some loss in chromatographic resolution, by use of an 80-μL NMR cell, Instead of a smaller 14-μL cell. Analysis of the antimycin A mixture, using the optimal analytical high performance liquid chromatography/nuclear magnetic resonance conditions, reveals it to consist of at least 10 closely related components.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Analysis of antimycin A by reversed-phase liquid chromatography/nuclear magnetic-resonance spectrometry
Series title:
Analytical Chemistry
Volume
61
Issue:
5
Year Published:
1989
Language:
English
Publisher:
ACS Publications
Publisher location:
Washington, D.C.
Contributing office(s):
Upper Midwest Environmental Sciences Center
Description:
5 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Analytical Chemistry
First page:
404
Last page:
408
Number of Pages:
5
Country:
United States