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Low-temperature incubation using a water supply

Applied Microbiology

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Abstract

Cell and tissue culture has been concerned primarily with homiothermic vertebrate cells which require incubation at about 37 C, and there is a great variety of incubators designed to maintain temperatures which are usually above ambient. The culture of poikilothermic vertebrate cells--and invertebrate, plant, and some microbial cells--can often be carried out at ambient temperatures, but for some work cooler conditions must be provided. Variety among the so-called low-temperature incubators is somewhat restricted; there are no small units, and all require a power source to maintain temperatures below ambient. We have used a gravity-fed water supply for 5 years to provide trouble-free, constant, low-temperature incubation of stock cultures of fish and amphibian cells. Though it is but a small part of our low-temperature incubator capacity, it has no power requirements and it provides maximal protection against temperature rises which could be lethal to some of the cell lines. Though the system has limitations, there is a considerable likelihood that the domestic water supply in other laboratories can also be used to provide low-temperature incubation.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Low-temperature incubation using a water supply
Series title:
Applied Microbiology
Volume
15
Issue:
6
Year Published:
1967
Language:
English
Publisher:
American Society for Microbiology
Publisher location:
Washington, D.C.
Contributing office(s):
Leetown Science Center
Description:
1 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Applied Microbiology
First page:
1501
Last page:
1501
Number of Pages:
1