| Abstract: | Cell and tissue culture has been concerned primarily with homiothermic vertebrate cells which require incubation at about 37 C, and there is a great variety of incubators designed to maintain temperatures which are usually above ambient. The culture of poikilothermic vertebrate cells--and invertebrate, plant, and some microbial cells--can often be carried out at ambient temperatures, but for some work cooler conditions must be provided. Variety among the so-called low-temperature incubators is somewhat restricted; there are no small units, and all require a power source to maintain temperatures below ambient. We have used a gravity-fed water supply for 5 years to provide trouble-free, constant, low-temperature incubation of stock cultures of fish and amphibian cells. Though it is but a small part of our low-temperature incubator capacity, it has no power requirements and it provides maximal protection against temperature rises which could be lethal to some of the cell lines. Though the system has limitations, there is a considerable likelihood that the domestic water supply in other laboratories can also be used to provide low-temperature incubation. |
| Genre: | Article |
| ProdID: | 70006948 |
| Citation Author: | Wolf, K.; Quimby, M. C. |
| Citation Contributing Office: | Leetown Science Center |
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| Citation End Page: | 1501 |
| Citation Issue: | 6 |
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| Citation Language: | English |
| Citation Larger Work Title: | Applied Microbiology |
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| Citation Number Of Pages: | 1 |
| Citation Online Only Flag: | |
| Citation Phsyical Description: | 1 p. |
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| Citation Public Comments: | None |
| Citation Publisher: | American Society for Microbiology |
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| Citation Search Results Text: | Low-temperature incubation using a water supply; 1967; Article; Journal; Applied Microbiology; Wolf, K.; Quimby, M. C. |
| Citation Start Page: | 1501 |
| Citation Volume: | 15 |
| Citation Year: | 1967 |
| Type: | citation/reference |
| Text: | Low-temperature incubation using a water supply; 1967; Article; Journal; Applied Microbiology; Wolf, K.; Quimby, M. C. |
| URL (DOCUMENT): | http://aem.asm.org/content/15/6/1501.full.pdf+html |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Sun, 1 Jan 2012 00:00 -0600 |
| Publisher: | American Society for Microbiology |
