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Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves

Journal of Wildlife Diseases

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Abstract

Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 104 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves
Series title:
Journal of Wildlife Diseases
Volume
48
Issue:
2
Year Published:
2012
Language:
English
Publisher:
Wildlife Disease Association
Publisher location:
Lawrence, KS
Contributing office(s):
Northern Prairie Wildlife Research Center
Description:
4 p.
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Wildlife Diseases
First page:
473
Last page:
476
Country:
United States
State:
Minnesota;Wyoming
Other Geospatial:
Superior National Forest;Yellowstone National Park