| Abstract: | The present study was initiated in order to develop a rapid and accurate method for the determination of uric acid in fresh, brackish, and sea water. It was found that the spectrophotometric determination of uric acid based upon its reaction with arsenophosphotungstic acid reagent in the presence of cyanide ion meets this objective. The absorbancy of the blue complex was measured at 890 m??. Slight variations from Beer‘s law were generally found. The results show the effects of pH, reaction time, concentration of reagents, and temperature upon color development and precipitate formation. Disodium dihydrogen ethylenediamine tetraacetate (Versene) was used as a buffering and complexirig agent. The results are significant in that they give the absorption spectrum of the blue complex and the effects of variables upon its absorbancy. Studies were made with the method to determine the stability of reagents and standard solutions and to determine the rate of bacterial decomposition of uric acid. Measurements of the solubility of uric acid are reported. |
| Genre: | Article |
| ProdID: | 70010590 |
| Citation Author: | Norton, D. R.; Plunkett, M. A.; Richards, F. A. |
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| Citation End Page: | 457 |
| Citation Issue: | 3 |
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| Citation Language: | English |
| Citation Larger Work Title: | Analytical Chemistry |
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| Citation Number Of Pages: | 4 |
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| Citation Search Results Text: | Spectrophotometric determination of uric acid and some redeterminations of its solubility; 1954; Article; Journal; Analytical Chemistry; Norton, D. R.; Plunkett, M. A.; Richards, F. A. |
| Citation Start Page: | 454 |
| Citation Volume: | 26 |
| Citation Year: | 1954 |
| Type: | citation/reference |
| Text: | Spectrophotometric determination of uric acid and some redeterminations of its solubility; 1954; Article; Journal; Analytical Chemistry; Norton, D. R.; Plunkett, M. A.; Richards, F. A. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Fri, 1 Jan 1954 00:00 -0600 |
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