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Spectrophotometric determination of uric acid and some redeterminations of its solubility

Analytical Chemistry

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Abstract

The present study was initiated in order to develop a rapid and accurate method for the determination of uric acid in fresh, brackish, and sea water. It was found that the spectrophotometric determination of uric acid based upon its reaction with arsenophosphotungstic acid reagent in the presence of cyanide ion meets this objective. The absorbancy of the blue complex was measured at 890 m??. Slight variations from Beer's law were generally found. The results show the effects of pH, reaction time, concentration of reagents, and temperature upon color development and precipitate formation. Disodium dihydrogen ethylenediamine tetraacetate (Versene) was used as a buffering and complexirig agent. The results are significant in that they give the absorption spectrum of the blue complex and the effects of variables upon its absorbancy. Studies were made with the method to determine the stability of reagents and standard solutions and to determine the rate of bacterial decomposition of uric acid. Measurements of the solubility of uric acid are reported.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Spectrophotometric determination of uric acid and some redeterminations of its solubility
Series title:
Analytical Chemistry
Volume
26
Issue:
3
Year Published:
1954
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Analytical Chemistry
First page:
454
Last page:
457
Number of Pages:
4