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A procedure for estimating Bacillus cereus spores in soil and stream-sediment samples - A potential exploration technique

Journal of Geochemical Exploration

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Abstract

The presence of bacterial spores of the Bacillus cereus group in soils and stream sediments appears to be a sensitive indicator of several types of concealed mineral deposits, including vein-type gold deposits. The B. cereus assay is rapid, inexpensive, and inherently reproducible. The test, currently under investigation for its potential in mineral exploration, is recommended for use on a research basis. Among the aerobic spore-forming bacilli, only B. cereus and closely related strains produce an opaque zone in egg-yolk emulsion agar. This characteristic, also known as the Nagler of lecitho-vitellin reaction, has long been used to rapidly indentify and estimate presumptive B. cereus. The test is here adapted to permit rapid estimation of B. cereus spores in soil and stream-sediment samples. Relative standard deviation was 10.3% on counts obtained from two 40-replicate pour-plate determinations. As many as 40 samples per day can be processed. Enough procedural detail is included to permit investigation of the test in conventional geochemical laboratories using standard microbiological safety precautions. ?? 1985.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A procedure for estimating Bacillus cereus spores in soil and stream-sediment samples - A potential exploration technique
Series title:
Journal of Geochemical Exploration
Volume
23
Issue:
3
Year Published:
1985
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Geochemical Exploration
First page:
243
Last page:
252
Number of Pages:
10