Transport of microspheres and indigenous bacteria through a sandy aquifer: Results of natural- and forced-gradient tracer experiments
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Abstract
Transport of indigenous bacteria through sandy aquifer sediments was investigated in forced- and natural-gradient tracer teste. A diverse population of bacteria was collected and concentrated from groundwater at the site, stained with a DNA-specific fluorochrome, and injected back into the aquifer. Included with the injectate were a conservative tracer (Br- or Cl-) and bacteria-sized (0.2-1.3-??m) microspheres having carboxylated, carbonyl, or neutral surfaces. Transport of stained bacteria and all types and size classes of microspheres was evident. In the natural-gradient test, both surface characteristics and size of microspheres affected attenuation. Surface characteristics had the greatest effect upon retardation. Peak break-through of DAPI-stained bacteria (forced-gradient experiment) occurred well in advance of bromide at the more distal sampler. Transport behavior of bacteria was substantially different from that of carboxylated microspheres of comparable size.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Transport of microspheres and indigenous bacteria through a sandy aquifer: Results of natural- and forced-gradient tracer experiments |
| Series title | Environmental Science & Technology |
| DOI | 10.1021/es00178a005 |
| Volume | 23 |
| Issue | 1 |
| Year Published | 1989 |
| Language | English |
| Publisher | ACS |
| Contributing office(s) | Toxic Substances Hydrology Program |
| Description | 6 p. |
| First page | 51 |
| Last page | 56 |
| Google Analytic Metrics | Metrics page |