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Application of a hollow-fiber, tangential-flow device for sampling suspended bacteria and particles from natural waters

Journal of Environmental Quality

By:
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Abstract

The design and application of a hollow-fiber tangential-flow filtration device has been used to concentrate bacteria and suspended particles from large volume surface water and groundwater samples (i.e., hundreds of liters). Filtrate flux rates (4-8 L min-1) are equal to or faster than those of other devices that are based on continuous flow centrifugation and plate and frame filtration. Particle recovery efficiencies for inorganic particles (approximately 90%) were similar to other dewatering devices, but microbial cell recoveries (30-90%) were greatly improved by this technique relative to other currently available methods. Although requirements for operation and maintenance of the device are minimal, its size, as with other dewatering devices, limits its applicability at remote sample sites. Nevertheless, it has proven useful for sample collection in studies involving microbial transport and analysis of particle-associated trace inorganic solutes.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Application of a hollow-fiber, tangential-flow device for sampling suspended bacteria and particles from natural waters
Series title:
Journal of Environmental Quality
Volume
19
Issue:
3
Year Published:
1990
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Environmental Quality
First page:
625
Last page:
629