| Abstract: | The dissimilatory Fe(III) reducer, GS-15, is the first microorganism known to couple the oxidation of aromatic compounds to the reduction of Fe(III) and the first example of a pure culture of any kind known to anaerobically oxidize an aromatic hydrocarbon, toluene. In this study, the metabolism of toluene, phenol, and p-cresol by GS-15 was investigated in more detail. GS-15 grew in an anaerobic medium with toluene as the sole electron donor and Fe(III) oxide as the electron acceptor. Growth coincided with Fe(III) reduction. [ring-14C]toluene was oxidized to 14CO2, and the stoichiometry of 14CO2 production and Fe(III) reduction indicated that GS-15 completely oxidized toluene to carbon dioxide with Fe(III) as the electron acceptor. Magnetite was the primary iron end product during toluene oxidation. Phenol and p-cresol were also completely oxidized to carbon dioxide with Fe(III) as the sole electron acceptor, and GS-15 could obtain energy to support growth by oxidizing either of these compounds as the sole electron donor. p-Hydroxybenzoate was a transitory extracellular intermediate of phenol and p-cresol metabolism but not of toluene metabolism. GS-15 oxidized potential aromatic intermediates in the oxidation of toluene (benzylalcohol and benzaldehyde) and p-cresol (p-hydroxybenzylalcohol and p-hydroxybenzaldehyde). The metabolism described here provides a model for how aromatic hydrocarbons and phenols may be oxidized with the reduction of Fe(III) in contaminated aquifers and petroleum-containing sediments. |
| Genre: | Article |
| ProdID: | 70016359 |
| Citation Author: | Lovley, D. R.; Lonergan, D. J. |
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| Citation End Page: | 1864 |
| Citation Issue: | 6 |
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| Citation Language: | English |
| Citation Larger Work Title: | Applied and Environmental Microbiology |
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| Citation Number Of Pages: | 7 |
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| Citation Search Results Text: | Anaerobic oxidation of toluene, phenol, and p-cresol by the dissimilatory iron-reducing organism, GS-15; 1990; Article; Journal; Applied and Environmental Microbiology; Lovley, D. R.; Lonergan, D. J. |
| Citation Start Page: | 1858 |
| Citation Volume: | 56 |
| Citation Year: | 1990 |
| Type: | citation/reference |
| Text: | Anaerobic oxidation of toluene, phenol, and p-cresol by the dissimilatory iron-reducing organism, GS-15; 1990; Article; Journal; Applied and Environmental Microbiology; Lovley, D. R.; Lonergan, D. J. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Mon, 1 Jan 1990 00:00 -0600 |
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