Enigmatic tubular trace fossils up to eight meters long occur in the Lower Pennsylvanian Middlesboro Member of the Lee Formation. Two morphotypes occur: type 1 trace fossils are plain, smooth, vertical, nonbranching, parallel-walled, tubular structures, type 2 trace fossils branch, have walls with faint vertical striations, regularly or irregularly spaced nodes, and funnel-shaped terminations. Sandstone casts filling type 2 structures have helical spiral morphology, and, in rare individuals, faint meniscate fills have been observed. Both trace-fossil morphotypes have poorly cemented wall linings containing framboidal pyrite, amorphous carbon, quartz sand, and poorly preserved fecal material. The trace fossils occur in a massive, structureless, channel-form sandstone, originating at the contact between a channel lag and the overlying massive fill representing a barrier island transgressing an estuarine facies. Origin of these structures is uncertain. -from Authors