In situ measurement of methane oxidation in groundwater by using natural-gradient tracer tests
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Abstract
Methane oxidation was measured in an unconfined sand and gravel aquifer (Cape Cod, Mass.) by using in situ natural-gradient tracer tests at both a pristine, oxygenated site and an anoxic, sewage-contaminated site. The tracer sites were equipped with multilevel sampling devices to create target grids of sampling points; the injectate was prepared with groundwater from the tracer site to maintain the same geochemical conditions. Methane oxidation was calculated from breakthrough curves of methane relative to halide and inert gas (hexafluoroethane) tracers and was confirmed by the appearance of 13C-enriched carbon dioxide in experiments in which 13C-enriched methane was used as the tracer. A Vmax for methane oxidation could be calculated when the methane concentration was sufficiently high to result in zero-order kinetics throughout the entire transport interval. Methane breakthrough curves could be simulated by modifying a one-dimensional advection-dispersion transport model to include a Michaelis-Menten-based consumption term for methane oxidation. The Km values for methane oxidation that gave the best match for the breakthrough curve peaks were 6.0 and 9.0 μM for the uncontaminated and contaminated sites, respectively. Natural-gradient tracer tests are a promising approach for assessing microbial processes and for testing in situ bioremediation potential in groundwater systems.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | In situ measurement of methane oxidation in groundwater by using natural-gradient tracer tests |
| Series title | Applied and Environmental Microbiology |
| DOI | 10.1128/aem.57.7.1997-2004.1991 |
| Volume | 57 |
| Issue | 7 |
| Year Published | 1991 |
| Language | English |
| Publisher | American Society for Microbiology |
| Contributing office(s) | Toxic Substances Hydrology Program |
| Description | 8 p. |
| First page | 1997 |
| Last page | 2004 |
| Country | United States |
| State | Massachusetts |
| Other Geospatial | Cape Cod |
| Google Analytic Metrics | Metrics page |